Improved FSH sensitisation and aromatase assay in human granulosa-lutein cells

Molecular Human Reproduction

Volumn 6, Issue 8, 2000, Pages 677-680

  Lambert, A ^a,b   Harris, S D ^a,c   Knaggs, P ^a,d   Robertson, W R ^a,b  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^c ROYAL MANCHESTER CHILDREN S HOSPITAL   (United Kingdom)

^d QUEEN S MEDICAL CENTRE   (United Kingdom)

Author keywords

Aromatase; FSH; Granulosa cells; Granulosa lutein cells

Indexed keywords

ANDROSTENEDIONE; AROMATASE; ESTROGEN; FOLLITROPIN; HYDROCORTISONE; PRASTERONE; PROGESTERONE; TESTOSTERONE;

ARTICLE; CELL CULTURE; ENZYME ACTIVITY; ENZYME ASSAY; ESTROGEN BLOOD LEVEL; FERTILIZATION IN VITRO; GRANULOSA CELL; HORMONAL REGULATION; HORMONE DETERMINATION; HUMAN; HUMAN CELL; LUTEAL CELL; PRIORITY JOURNAL; SENSITIZATION; TESTOSTERONE BLOOD LEVEL;

EID: 0033870137     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/6.8.677     Document Type: Article

References (11)

1. Effect of insulin-like growth factor-1 on steroidogenesis in cultured human granulosa cells
2. Recombinant human gonadotrophins stimulate steroid and inhibin production in human granulosa cells
3. Insulin-like growth factor-I regulates aromatase activity in human granulosa and granulosa luteal cells
4. Progesterone production by human granulosa cells cultured in serum free medium: Effects of gonadotrophins and insulin-like growth factor-I (IGF-1)
5. Oestradiol production by luteinized human granulosa cells: Evidence of the stimulatory action of recombinant follicle-stimulating hormone
6. In-vitro biopotency of two glycoform mixture of differing acidity derived from recombinant FSH measured using a human granulosa-lutein cell bioassay
7. In vitro recovery of human chorionic gonadotrophin-stimulated cyclic adenosine 3', 5'-monophosphate production in desensitised human granulosa-luteal cells
8. A rapid (<8 h) method for the measurement of the oxoreductase activity of 11β-hydroxysteroid dehydrogenase in granulosa-lutein cells from patients undergoing IVF
9. Exogenous steroids and the control of oestradiol secretion by human granulosa-lutein cells by follicle stimulating hormone and insulin-like growth factor-I
10. A sensitive and specific in vitro bioassay method for the determination of FSH activity
11. Hormonal regulation of the growth and steroidogenic function of human granulosa cells