The dihydrolipoyl acyltransferase (BCE2) subunit of the plant branched- chain α-ketoacid dehydrogenase complex forms a 24-mer core with octagonal symmetry

Protein Science

Volumn 9, Issue 7, 2000, Pages 1334-1339

  Mooney, Brian P ^a   Henzl, Michael T ^a   Miernyk, Jan A ^a,b   Randall, Douglas D ^a,c  

^a University of Missouri   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

^c UNIVERSITY OF MISSOURI   (United States)

Author keywords

24 mer cubic core; Arabidopsis thaliana; Dihydrolipoyl acyltransferase; Recombinant expression

Indexed keywords

2 OXOACID DEHYDROGENASE; ACYLTRANSFERASE;

ANALYTIC METHOD; ARABIDOPSIS; ARTICLE; ENZYME ANALYSIS; ENZYME STRUCTURE; ENZYME SUBUNIT; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STABILITY; TRANSMISSION ELECTRON MICROSCOPY;

ESCHERICHIA COLI;

EID: 0033868183     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.7.1334     Document Type: Article

References (30)

1. Ævarsson A, Seger K, Turley S, Sokatch JR, Hol WG. 1999. Crystal structure of the 2-oxoisovalerate dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes. Nat Struct Biol 6:785-792.
2. Behal RH, DeBuysere MS, Demeler B, Hansen JC, Olson MS. 1994. Pyruvate dehydrogenase multienzyme complex. J Biol Chem 269:31372-31377.
3. Bosma HJ, De Kok A, Van Markwijk BW, Veeger C. 1984. The size of the pyruvate dehydrogenase complex of Azotobacter vinelandii. Eur J Biochem 140:273-280.
4. Chuang DT. 1988. Assays for E1 and E2 components of the branched-chain keto acid dehydrogenase complex. Methods Enzymol 166:146-154.
5. Danner DJ, Litwer S, Herring WJ, Pruckler J. 1989. Construction and nucleotide sequence of a cDNA encoding the full-length preprotein for human branched chain acyltransferase. J Biol Chem 264:7742-7746.
6. De Marcucci OL, DeBuysere MS, Olson MS. 1995. Dissociation and reassembly of the dihydrolipoyl transacetylase component of the bovine heart pyruvate dehydrogenase complex. Arch Biochem Biophys 323:169-176.
7. Griffin TA, Lau KS, Chuang DT. 1988. Characterization and conservation of the inner E2 core domain structure of branched-chain α-keto acid dehydrogenase complex from bovine liver. J Biol Chem 263:14008-140014.
8. Griffin TA, Wynn RM, Chuang DT. 1990. Expression and assembly of mature apotransacylase (E2b) of bovine branched-chain α-keto acid dehydrogenase complex in Escherichia coli. J Biol Chem 265:12104-12110.
9. Guest JR, Angier SJ, Russell GC. 1989. Structure, expression and protein engineering in the pyruvate dehydrogenase complex of Escherichia coli. J Mol Biol 185:743-754.
10. Koike M, Reed LJ, Carrol WR. 1963. α-Keto acid dehydrogenase complexes. IV. Resolution and reconstitution of the Escherichia coli pyruvate dehydrogenase complex. J Biol Chem 238:30-39.
11. Lau KS, Phillips CE, Randle PJ. 1983. Multi-site phosphorylation in ox-kidney branched-chain 2-oxoacid dehydrogenase complex. FEBS Lett 160:149-152.
12. Lessard IAD, Domingo GJ, Borges A, Perham RN. 1998. Expression of genes encoding the E2 and E3 components of the Bacillus sterothermophilus pyruvate dehydrogenase complex and the stoichiometry of subunit interactions in assembly in vitro. Eur J Biochem 258:491-501.
13. Luethy MH, Miernyk JA, Randall DD. 1998. The nucleotide sequence of a cDNA encoding the E1-beta subunit of the branched-chain alpha-keto acid dehydrogenase from Arabidopsis thaliana (Accession No. AF061638). (PGR98-133) Plant Physiol 118:329.
14. Madhusudhan KT, Luo J, Sokatch JR. 1999. In vitro transcriptional studies of the bkd operon of Psuedomonas putida: L-Branched-chain amino acids and D-leucine are the inducers. J Bacteriol 181:2889-2894.
15. Mattevi A, Obmolova G, Schulze E, Kalk KH, Westphal AH, de Kok A, Hol WG. 1992. Atomic structure of the cubic core of the pyruvate dehydrogenase multienzyme complex. Science 255:1544-1550.
16. Miernyk JA. 1999. Protein folding in the plant cell. Plant Physiol 121:695-703.
17. Mooney BP, Miernyk JA, Randall DD. 1998a. Nucleotide sequence of a cDNA encoding the dihydrolipoyl acyltransferase (E2) subunit of the branched-chain α-keto acid dehydrogenase complex from Arabidopsis thaliana (Accession No. AF038505) (PGR 98-071). Plant Physiol 117:331.
18. Mooney BP, Miernyk JA, Randall DD. 1998b. Nucleotide sequence of a cDNA encoding the E1α subunit of the branched-chain α-keto acid dehydrogenase complex from Arabidopsis thaliana (Accession No. AF077955) (PGR98-168). Plant Physiol 118:711.
19. Mooney BP, Miernyk JA, Randall DD. 1999. Cloning and characterization of the dihydrolipoamide S-acetyltransferase subunit of the plastid pyruvate dehydrogenase complex (E2) from Arabidopsis. Plant Physiol 120:443-452.
20. Oliver RM, Reed LJ. 1982. Multienzyme complexes. In: Harris JR, ed. Electron microscopy of proteins. London, UK: Academic Press, pp 1-48.
21. Patel MS, Roche TE. 1990. Molecular biology and biochemistry of pyruvate dehydrogenase complexes. FASEB J 4:3224-3233.
22. Philo JS. 1994. Measuring sedimentation, diffusion, and molecular weights of small molecules by direct fitting of sedimentation velocity concentration profiles. In: Schuster TM, Laue TM, eds. Modern analytical ultracentrifugation. Boston, MA: Birkhauser. pp 156-170.
23. Reed LJ. 1974. Multienzyme complexes. Acc Chem Res 7:40-46.
24. Reed LJ, Hackert ML. 1990. Structure-function relationships in dihydrolipoamide acyltransferases. J Biol Chem 265:5971-5974.
25. Sjöling S, Glaser E. 1998. Mitochondrial targeting peptides in plants. Trends Plant Sci 3:136-140.
26. Thelen JJ, Muszynski MG, David NR, Luethy MH, Elthon TE, Miernyk JA, Randall DD. 1999. The dihydrolipoamide S-acetyltransferase subunit of the mitochondrial pyruvate dehydrogenase complex from maize contains a single lipoyl domain. J Biol Chem 274:21769-21775.
27. Ward DE, Ross RP, van der Weijden CC, Snoep JL, Claiborne A. 1999. Catabolism of the branched-chain α-keto acids in Enterococcus faecalis: The bkd gene cluster, enzymes, and metabolic route. J Bacteriol 181:5433-5442.
28. Wynn RM, Davie JR, Zhi W, Cox RP, Chuang DT. 1994. In vitro reconstitution of the 24-meric E2 inner core of bovine mitochondrial branched-chain α-keto acid dehydrogenase complex: Requirement for chaperonins GroEL and GroES. Biochemistry 33:8962-8968.
29. Yang D, Song J, Wagenknecht T, Roche TE. 1997. Assembly and full functionality of recombinantly expressed dihydrolipoyl acetyltransferase component of the human pyruvate dehydrogenase complex. J Biol Chem 272:6361-6369.
30. Yeaman SJ. 1989. The 2-oxoacid dehydrogenase complexes: Recent advances. Biochem J 257:625-632.