Plasminogen activator inhibitor-1 fused with erythropoietin (EPO) mimetic peptide (EMP) enhances the EPO activity of EMP

Journal of Peptide Research

Volumn 56, Issue 2, 2000, Pages 59-62

  Kuai, Le Tian ^a   Wu, Chun Lei ^a   Qui, Qi Feng ^a   Zhang, Jing ^a   Zhou, Ai Wu ^a   Wang, Shi Quan ^a   Zhang, He Yun ^a   Song, Qian ^a   Liao, Shu Ning ^a   Han, Yu ^a   Liu, Jian Ning ^a   Ma, Zhong ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

EMP; EPO activity; Fusion; PAI 1

Indexed keywords

ALANINE; ERYTHROPOIETIN; PLASMINOGEN ACTIVATOR INHIBITOR 1; PROLINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; GENE INSERTION; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RETICULOCYTE COUNT;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROPOIETIN; ESCHERICHIA COLI; MOLECULAR MIMICRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PLASMINOGEN ACTIVATOR INHIBITOR 1;

EID: 0033866583     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3011.2000.00716.x     Document Type: Article

References (11)

1. 1. Livnah, O. (1996) Functional memory of a protein hormone by a peptide agonist: the EPO receptor complex at 2.8 Å. Science 273, 464-470.
2. 2. Wrighton, N.C. (1996) Small peptides as potent mimetics of the protein hormone erythropoietin. Science 273, 458-463.
3. 3. Wells, J.A. (1996) Hormone mimiery. Science 273, 449-450.
4. 4. Wrighton, N.C. (1997) Increased potency of an erythropoietin peptide mimetic through covalent dimerization. Nat. Biotechnol. 15, 1261-1265.
5. 5. Derman, A.I. (1993) Mutations that allow disulfide bond formation in the cytoplasm of Escherichia coli. Science 262, 1744-1747.
6. 6. Ausubel, F.M. (1993) Current Protocols in Molecular Biology. Wiley, New York, pp. 10.11.8-10.11.22.
7. 7. Sambrook, J. (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 880-890.
8. 8. Hayakawa, T. (1992) Simple in vivo bioassay without radioisotopes for recombinant human erythropoientins. Biologicals 20, 243-251.
9. 9. Wang, Q. (1997) Establishment of reticulocyte counting method used for the detection of biological activity in vivo of EPO. J. Chinese Biol. Products 10, 45-48.
10. 10. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
11. 11. Mottonen, J. (1992) Structural basis of latency in plasminogen activator inhihitor-1. Nature 355, 270-273.