Expression of E1 component of human branched-chain α-keto acid dehydrogenase complex in Escherichia coli by cotransformation with chaperonins GroEL and GroES

Methods in Enzymology

Volumn 324, Issue , 2000, Pages 179-191

  Wynn, R Max ^a   Davie, James R ^a   Song, Jiu Li ^a   Chuang, Jacinta L ^a   Chuang, David T ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONIN; MALTOSE BINDING PROTEIN; OXIDOREDUCTASE;

ARTICLE; COMPLEX FORMATION; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0033858901     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (11)

1. A. Ævarsson, K. Seger, S. Turley, J. R. Sokatch, and W. G. J. Hol, Nature Struct. Biol. 6, 785 (1999).
2. A. Ævarsson, J. L. Chuang, R. M. Wynn, S. Turley, D. T. Chuang, and W. G. J. Hol, Structure 8, 277 (2000).
3. D. T. Chuang and V. E. Shih, "The Metabolic and Molecular Basis of Inherited Disease," 7th Ed, p. 1239. McGraw-Hill, New York, 1995.
4. R. M. Wynn, J. L. Chuang, J. R. Davie. C. W. Fisher, M. A. Hale, R. P. Cox, and D. T. Chuang, J. Biol. Chem. 267, 1881 (1992).
5. O. K. Kellerman and T. Ferenci, Methods Enzymol. 90, 459 (1982).
6. J. Sambrook, E. F. Fritsch, and T. Maniatis, "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989.
7. K. S. Lau, A. J. L. Cooper, and D. T. Chuang, Biochim. Biophys. Acta 1038, 360 (1990).
8. R. M. Wynn, J. R. Davie, J. L. Chuang, C. D. Cote, and D. T. Chuang, J. Biol. Chem. 273, 13110 (1998).
9. J. L. Chuang, R. M. Wynn, J.-L. Song, and D. T. Chuang, J. Biol. Chem. 274, 10395 (1999).
10. L. M. Guzman-Verduzco and Y. M. Kupersztoch, J. Bacteriol. 169, 5201 (1987).
11. J. L. Chuang, J. R. Davie, R. M. Wynn, and D. T. Chuang, Methods Enzymol. 324, Chap. 19, 2000 (this volume).