RNA amplification technique, NASBA, also amplifies homologous plasmid DNA in non-denaturing conditions

BioTechniques

Volumn 29, Issue 2, 2000, Pages 236-240

  Voisset, C ^a   Mandrand, B ^a   Paranhos Baccala, G ^a  

^a UNIVERSITÉ DE LYON   (France)

Author keywords

[No Author keywords available]

Indexed keywords

PLASMID DNA; RNA; RNA POLYMERASE; VIRUS RNA;

ARTICLE; COLORIMETRY; GENE AMPLIFICATION; GENETIC TRANSCRIPTION; NONHUMAN; NUCLEOTIDE SEQUENCE; RETROVIRUS; RNA HYBRIDIZATION; RNA SEQUENCE; SEQUENCE HOMOLOGY; STRUCTURAL GENE; VIRUS GENOME;

UNIDENTIFIED RETROVIRUS;

EID: 0033848507     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00292bm07     Document Type: Article

References (7)

1. Molecular characterization and placental expression of HERV-W, a new human endogenous retrovirus family
2. Nucleic acid sequence-based amplification
3. Detection of virion-associated MSRV-RNA in serum of patients with multiple sclerosis
4. Highly sensitive detection of gene expression of an intronless gene: Amplification of mRNA, but not genomic DNA by nucleic acid sequence based amplification (NASBA)
5. NASBA isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnostis of HIV-1 infection
6. The viruses in all of us: Characteristics and biological significance of human endogenous retroviral sequences
7. Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis