The Neurospora am gene and NADP-specific glutamate dehydrogenase: Mutational sequence changes and functional effects - More mutants and a summary

Genetical Research

Volumn 76, Issue 1, 2000, Pages 1-10

  Fincham, J R S ^a   Kinsey, J A ^b   Fuentes, A M ^c   Cummings, N J ^c   Connerton, I F ^c  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GLUTAMATE DEHYDROGENASE (NADP);

ALLELE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; GENE FUNCTION; GENE MUTATION; GENETIC CODE; GENETIC COMPLEMENTATION; NEUROSPORA; NONHUMAN; REVERTANT;

ALLELES; AMINO ACIDS; DNA MUTATIONAL ANALYSIS; GENES, FUNGAL; GENETIC COMPLEMENTATION TEST; GENOTYPE; GLUTAMATE DEHYDROGENASE (NADP+); GLUTAMIC ACID; MUTATION; NEUROSPORA; NUCLEIC ACID CONFORMATION; NUCLEOTIDES; PHENOTYPE; PROTEIN CONFORMATION; SPECTROPHOTOMETRY; TIME FACTORS;

FUNGI; MYXOGASTRIA; NEUROSPORA;

EID: 0033846090     PISSN: 00166723     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0016672300004602     Document Type: Article

References (33)

1. Slow conformational changes of a Neurospora glutamate dehydrogenase studied by protein fluorescence
2. Mutational variants of Neurospora crassa NADP-specific glutamate dehydrogenase altered in a conformational equilibrium
3. Subunit assembly and active site location in the structure of glutamate dehydrogenase
4. Mutational amino acid replacements in Neurospora crassa NADP-specific glutamate dehydrogenase
5. Structural relationships between the hexameric and tetrameric families of glutamate dehydrogenase
6. Sequencing studies of IC-170 mutagenic specificity in the am (NADP-specific glutamate dehydrogenase) gene of Neurospora crassa
7. Multiple active forms of Neurospora glutamate dehydrogenase formed by hybridisation between two inactive mutant proteins in vivo and in vitro
8. A modified glutamic dehydrogenase as a result of gene mutation in Neurospora crassa
9. The role of chromosomal loci in enzyme formation
10. Genetically determined multiple form of glutamate dehydrogenase in Neurospora crassa
11. Recombination within the am gene of Neurospora crassa
12. A gene-enzyme relationship in Neurospora
13. The Neurospora am gene and allelic complementation
14. Generation of new functional mutant alleles by premeiotic disruption of the Neurospora crassa am gene
15. The molecular basis of an osmotically reparable mutant of Neurospora crassa producing unstable glutamate dehydrogenase
16. A further genetic variety of glutamic acid dehydrogenase in Neurospora crassa
17. Complementation relationships of Neurospora mutants in relation to their formation of abnormal forms of glutamate dehydrogenase
18. The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene
19. Alternative modes of mRNA processing in a 3' splice site mutant of Neurospora crassa
20. Direct selective procedure for isolating Neurospora mutants defective in NADP-specific glutamate dehydrogenase
21. Mutation at the am locus of Neurospora crassa
22. New mutational varieties of Neurospora NADP-specific glutamate dehydrogenase
23. Complementation and enzyme studies of revertants induced in an am mutant of Neurospora crassa
24. Fine structure map of the am (GDH) locus of Neurospora
25. An unstable mutant gene of the am locus of Neurospora results from a small duplication
26. Amino acid replacements resulting from suppression and mis-sense reversion of a chain terminator mutation in Neurospora
27. Frameshift mutations affecting the N-terminal sequence of Neurospora NADP-specific glutamate dehydrogenase
28. An easy method for preparing Neurospora DNA
29. Conformational flexibility in glutamate dehydrogenase: Role of water in substrate recognition and catalysis
30. Insights into the mechanism of domain closure and substrate specificity of glutamate dehydrogenase from Clostridium symbiosum
31. A mutant enzyme of Neurospora crass interconvertible between electrophoretically distinct active and inactive forms
32. Subunit ratios of separated hybrid hexamers of Neurospora NADP-specific glutamate dehydrogenase containing complementing mutationally modified monomers
33. Allosteric effects in NADP-specific glutamate dehydrogenase from Neurospora