Reduced ammonium chloride haemolysis time enhances the number of isolated functional rabbit polymorphonuclear neutrophils

APMIS

Volumn 108, Issue 6, 2000, Pages 417-421

  Kouoh, Ferdinand ^a   Levert, Helene ^a   Gressier, Bernard ^a,b   Luyckx, Michel ^a   Brunet, Claude ^a   Dine, Thierry ^a   Ballester, Louis ^a   Cazin, Micheline ^a   Cazin, Jean Claude ^a  

^a UNIV LILLE   (France)

^b Pharmacocinétique et Pharmacie Clinique   (France)

Author keywords

Ammonium chloride haemolysis; Histopaque ; Percoll ; Rabbit PMNs; Respiratory burst.

Indexed keywords

AMMONIUM CHLORIDE; DEXTRAN; PHORBOL 13 ACETATE 12 MYRISTATE; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL ISOLATION; CELL VIABILITY; CONTROLLED STUDY; ERYTHROCYTE; HEMOLYSIS; LYMPHOCYTE; MALE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; RABBIT; RESPIRATORY BURST; TIME;

EID: 0033837893     PISSN: 09034641     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0463.2000.d01-77.x     Document Type: Article

References (22)

1. The respiratory burst of phagocytic leucocytes
2. Oxidants from phagocytes: Agents of defense and destruction
3. Isolation of mononuclear cells and granulocytes from human blood
4. Rapid method for the isolation of neutrophils in high yield without the use of dextran or density gradient polymers
5. A rapid density gradient technique for separation of polymorphonuclear granulocytes
6. Separation of neutrophils from blood in human and laboratory animals and comparison of the chemotaxis
7. Activation et toxicite de l'oxygene. Principes des therapeutiques antioxydantes
8. Oxidative stress: From basic research to clinical application
9. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease
10. Superoxide generation by digitonin-stimulated guinea pig granulocytes. A basis for continuous assay for monitoring superoxide production and for study of the activation of the generating system
11. Azithromycin impact on neutrophil oxidative metabolism depends on exposure time
12. A simple colorimetric method for the measurement of hydrogen peroxide production by cells in culture
13. In vitro interaction between dirithromycin and its metabolite, erythromycylamine, and oxidative polymorphonuclear metabolism
14. Tissue destruction by neutrophils
15. Separation of leucocytes: Improved cell purity by fine adjustments of gradient medium density and osmolality
16. Use of Histopaque for isolating mononuclear cells from rabbit blood
17. A rapid method for purification of human granulocytes using percoll. A comparison with dextran sedimentation
18. The decline of catalytic enzyme activity concentration of in vivo aging erythrocytes of the man, the dog and the rat. Approach to a quantitative diagnostic enzymology. IV. Communication
19. A single-step centrifugation method for separation of granulocytes and mononuclear cells from blood using discontinuous density gradient of percoll
20. Differences and similarities between human and rabbit neutrophil granulocyte responses in vitro: The effects of zymosan-activated plasma, phorbol myristate acetate and n-formyl-methionyl-leucyl-phenylalanine
21. Separation of lymphocytes, granulocytes, and monocytes from human blood using iodinated density gradient media
22. Degranulation enhances release of a stable contractile factor from rabbit polymorphonuclear leukocytes