Escherichia coli thymidylate synthase expression protects human cells from the cytotoxic effects of 5-fluorodeoxyuridine more effectively than human thymidylate synthase overexpression

Cancer Gene Therapy

Volumn 7, Issue 8, 2000, Pages 1179-1187

  Parsels, Leslie A ^a   Kowalski, Michael P ^b   Wagner, Lois M ^a   Parsels, Joshua D ^a   Maley, Frank ^c   Maybaum, Jonathan ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b HARVARD UNIVERSITY   (United States)

^c WADSWORTH CENTER   (United States)

Author keywords

5 Fluorodeoxyuridine; Host protection; Thymidylate synthase

Indexed keywords

BACTERIAL ENZYME; FLOXURIDINE; FOLINIC ACID; THYMIDYLATE SYNTHASE;

ARTICLE; CANCER CHEMOTHERAPY; CELL PROTECTION; CLONOGENESIS; CONTROLLED STUDY; DRUG CYTOTOXICITY; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; GENE OVEREXPRESSION; GROWTH INHIBITION; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SPECIES DIFFERENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0033837350     PISSN: 09291903     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cgt.7700221     Document Type: Article

References (32)

1. Gene therapy utilizing drug resistance genes: A review
2. Gene transfer of drug resistance genes: Implications for cancer therapy
3. Long term protection of recipient mice from lethal doses of methotrexate by marrow infected with a double copy vector retrovirus containing a mutant dihydrofolate reductase
4. Fluorinated pyrimidines and their nucleosides
5. The effect of methotrexate on levels of dUTP in animal cells
6. Methotrexate-induced misincorporation of uracil into DNA
7. DNA fragmentation and cytotoxicity from increased cellular deoxyuridylate
8. Antifolate-induced misincorporation of deoxyuridine monophosphate into DNA: Inhibition of high molecular weight DNA synthesis in human lymphoblastoid cells
9. Experimental chemotherapy of neuroblastoma: Increased thymidylate synthetase activity in a 5-fluorodeoxyuridine-resistant variant of mouse neuroblastoma
10. Increased thymidylate synthetase in 5-fluorodeoxyuridine-resistant cultured hepatoma cells
11. Thymidylate synthase gene amplification in human colon cancer cell lines resistant to 5-fluorouracil
12. Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors
13. Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase
14. Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells
15. Construction and use of a safe and efficient amphotropic packaging cell line
16. A safe packaging line for gene transfer: Separating viral genes on two different plasmids
17. Fluorouracil and high-dose leucovorin in previously treated patients with metastatic breast cancer
18. Complete restoration of activity to inactive mutants of Escherichia coli thymidylate synthase: Evidence that E. coli thymidylate synthase is a half-the-sites activity enzyme
19. Biochemical determinants of tumor sensitivity to 5-fluorouracil: Ultrasensitive methods for the determination of 5-fluoro-2'-deoxyuridylate, 2'-deoxyuridylate, and thymidylate synthetase
20. Rapid determination of thymidylate synthase activity and its inhibition in intact L1210 leukemia cells in vitro
21. Isolation and characterization of a human ileocecal carcinoma cell line (HCT-8) subclone resistant to fluorodeoxyuridine
22. Determination of 5-fluorouracil and its main metabolites in plasma by high-performance liquid chromatography
23. Activity of thymidylate synthetase and its inhibition by 5-fluorouracil in highly enzyme-overproducing cells resistant to 10-propargyl-5,8-dideazafolate
24. Unusual aspects of human thymidylate synthase in mouse cells introduced by DNA-mediated gene transfer
25. Folinic acid augmentation of the effects of fluoropyrimidines on murine and human leukemic cells
26. Catalytic role of histidine 147 in Escherichia coli thymidylate synthase
27. Substitution of glutamine for glutamic acid-58 in Escherichia coli thymidylate synthase results in pronounced decreases in catalytic activity and ligand binding
28. Variation in human thymidylate synthase is associated with resistance to 5-fluoro-2'-de-oxyuridine
29. Functional effects of a naturally occurring amino acid substitution in human thymidylate synthase
30. Factors affecting substrate binding in Lactobacillus casei thymidylate synthetase as studied by equilibrium dialysis
31. Biochemical factors affecting the tightness of 5-fluorodeoxyuridylate binding to human thymidylate synthetase
32. Retroviral coexpression of thymidylate synthase and dihydrofolate reductase confers fluoropyrimidine and antifolate resistance