Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid

Journal of Clinical Microbiology

Volumn 38, Issue 9, 2000, Pages 3214-3218

  Hukkanen, V ^a   Rehn, T ^a   Kajander, R ^a   Sjoroos, M ^a   Waris, M ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

EUROPIUM; LANTHANIDE; SAMARIUM; VIRUS GLYCOPROTEIN;

ARTICLE; CEREBROSPINAL FLUID CULTURE; FLUOROMETRY; HERPES SIMPLEX VIRUS; HERPES SIMPLEX VIRUS 1; HERPES SIMPLEX VIRUS 2; HUMAN; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIGNAL NOISE RATIO; SOUTHERN BLOTTING; VIRUS DETECTION; VIRUS ENCEPHALITIS; VIRUS MENINGITIS;

DNA VIRUSES; HERPES; HUMAN HERPESVIRUS 1; HUMAN HERPESVIRUS 2; SIMPLEXVIRUS;

EID: 0033823663     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.9.3214-3218.2000     Document Type: Article

References (26)

1. Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid
2. Time-resolved fluorometry for the identification of viral DNA in clinical specimens
3. Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time-resolved fuorescence-based hybridization assay
4. Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification
5. Detection of varicella-zoster virus-specific DNA sequences in cerebrospinal fluid from patients with acute aseptic meningitis and no cutaneous lesions
6. Detection of enteroviruses and rhinoviruses in clinical specimens by polymerase chain reaction and liquid-phase hybridization
7. Digoxigenin labeled probes and their use in the laboratory diagnosis of virus infections
8. Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry
9. Two-centre study comparing DNA preparation and PCR amplification protocols for herpes simplex virus detection in cerebrospinal fluids of patients with suspected herpes simplex encephalitis
10. Preparation of europium-labelled DNA probes and their properties
11. Detection of amplified HTLV-I/-II viral sequences using time-resolved fluorometry
12. Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction
13. Diagnosis of herpes simplex encephalitis: Application of polymerase chain reaction to cerebrospinal fluid from brain-biopsied patients and correlation with disease
14. Diagnosis of enterovirus and rhinovirus infections by RT-PCR and time-resolved fluorometry with lanthanide chelate labeled probes
15. Detection of lanthanide chelates by time-resolved fluorescence
16. Laboratory diagnosis of central nervous system infections with herpes simplex virus by PCR performed with cerebrospinal fluid specimens
17. Evaluation of the polymerase chain reaction for diagnosis of herpes simplex virus encephalitis
18. Establishment of PCR for the early diagnosis of herpes simplex encephalitis
19. Rapid detection of herpes simplex virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis
20. Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples
21. Time-resolved fluorometry based sandwich hybridisation assay for HLA-DQA1 typing
22. Time-resolved fluorescence of lanthanide probes and applications in biotechnology
23. Construction of europium (Eu 3+)-labelled oligo DNA hybridization probes
24. Molecular diagnosis of herpes simplex virus infections in the central nervous system
25. Detection of herpes simplex virus DNA in cerebrospinal fluid samples using the polymerase chain reaction and microplate hybridization
26. Herpes simplex virus detection by macroscopic reading after overnight incubation and immunoperoxidase staining