Isolation of full-length cDNA clones using SMART(TM) cDNA and a biotin-streptavidin bead system

BioTechniques

Volumn 29, Issue 3, 2000, Pages 444-450

  Ciavatta, V ^a   Cairney, J ^a  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; COMPLEMENTARY DNA; DNA FRAGMENT; MICROSATELLITE DNA; NUCLEIC ACID; STREPTAVIDIN;

ARTICLE; BIOTINYLATION; DNA HYBRIDIZATION; DNA PURIFICATION; DNA SYNTHESIS; EXPRESSED SEQUENCE TAG; GENE AMPLIFICATION; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; PINE; POLYMERASE CHAIN REACTION;

EID: 0033822677     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00293bm08     Document Type: Article

References (10)

1. Natural and somatic embryo development in loblolly pine: Gene expression studies using differential display and DNA arrays
2. Selection of hybrids by affinity capture (SHAC): A method for the generation of cDNAs enriched in sequences from a specific chromosome region
3. Genomic sequencing
4. Microsatellite enrichment in organisms with large genomes (Allium cepa L.)
5. Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer
6. Cloning of murine LAG-3 by magnetic bead bound homologous probes and PCR (GENE-CAPTURE PCR)
7. An embryo staging system for comparison of zygotic and somatic embryo development
8. Magnetic bead capture of cDNAs from double-stranded plasmid cDNA libraries
9. Rapid and reliable differential display results from minute amounts of tissue: Mass cloning and characterization of differentially expressed genes from loblolly pine embryos