Detection of p56(lck) kinase activity using scintillation proximity assay in 384-well format and imaging proximity assay in 384- and 1536-well format

Journal of Biomolecular Screening

Volumn 5, Issue 4, 2000, Pages 205-211

  Beveridge, Maxine ^a   Park, Young Whan ^b   Hermes, Jeff ^b   Marenghi, Angela ^a   Brophy, Gerard ^a   Santos, Albert ^a  

^a GENERAL ELECTRIC COMPANY   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; PEPTIDE; PHOSPHATE; PHOSPHOTRANSFERASE INHIBITOR; PROTEIN P56; REAGENT; STREPTAVIDIN;

ANALYTIC METHOD; ARTICLE; COST CONTROL; DEVICE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; IMAGING; IMAGING SYSTEM; INTERMETHOD COMPARISON; LYMPHOCYTE DIFFERENTIATION; PHOSPHATE TRANSPORT; PRIORITY JOURNAL; SCINTILLATION CAMERA; SCINTILLATION COUNTING; T LYMPHOCYTE ACTIVATION;

EID: 0033822413     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705710000500403     Document Type: Article

References (12)

1. T cell antigen receptor activation pathways: The tyrosine kinase connection
2. A lymphocyte-specific protein tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA
3. Expression of a new tyrosine protein kinase is stimulated by retrovirus promotor insertion
4. Transcriptional activation of lck by retrovirus promotor insertion between two lymphoid-specific promoters
5. The lymphocyte-specific tyrosine protein kinase p56(lck)
6. Genetic evidence for the involvement of the lck tyrosine kinase in signal transduction through the T cell antigen receptor
7. Scintillation proximity assay - A versatile high throughput screening technology
8. Imaging proximity assays
9. Site specificity of p72(syk) protein tyrosine kinase: Efficient phosphorylation of motifs recognised by Src homology 2 domains of the Src family
10. Catalytic specificity of protein-tyrosine kinases is critical for selective signalling
11. Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor
12. Nocodazole inhibits signal transduction by the T cell antigen receptor