Two-point fluorescence detection and automated fraction collection applied to constant denaturant capillary electrophoresis

BioTechniques

Volumn 29, Issue 3, 2000, Pages 582-589

  Ekstrom, P O ^a   Wasserkort, R ^a   Minarik, M ^a   Foret, F ^a   Thilly, W G ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; AUTOMATION; CAPILLARY ELECTROPHORESIS; DNA DETERMINATION; DNA SEQUENCE; FLUORESCENCE; POINT MUTATION; POLYMERASE CHAIN REACTION;

EID: 0033818736     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00293rr01     Document Type: Article

References (22)

1. Fidelity and mutational spectrum of Pfu DNA polymerase on a human mitochondrial DNA sequence
2. Mutation analyses of KRAS exon 1 comparing three different techniques: Temporal temperature gradient electrophoresis, constant denaturant capillary electrophoresis and allele specific polymerase chain reaction
3. Screening for germ line TP53 mutations in breast cancer patients
4. Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations
5. Detection of low-frequency mutations in exon 8 of the TP53 gene by constant denaturant capillary electrophoresis (CDCE)
6. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: Correspondence with melting theory
7. A simple method for identification of point mutations using denaturing gradient gel electrophoresis
8. High-performance electrophoresis elimination of electroendosmosis and solute adsorption
9. Constant denaturant gel electrophoresis, a modification of denaturing gradient gel electrophoresis, in mutation detection
10. Efficiency of separation of DNA mutations by constant denaturant capillary electrophoresis is controlled by the kinetics of DNA melting equilibrium
11. Identification of point mutations in mixtures by capillary electrophoresis hybridization
12. Constant denaturant capillary electrophoresis (CDCE): A high resolution approach to mutational analysis
13. The use of elevated column temperature to extend DNA sequencing read lengths in capillary electrophoresis with replaceable polymer matrices
14. Separation of transforming amino acid-substituting mutations in codons 12, 13 and 61 the N-ras gene by constant denaturant capillary electrophoresis (CDCE)
15. Use of wide-bore capillaries in constant denaturant capillary electrophoresis
16. Applications of constant denaturant capillary electrophoresis/high-fidelity polymerase chain reaction to human genetic analysis
17. Fraction collection in micropreparative capillary zone electrophoresis and capillary isoelectric focusing
18. Design of a high-precision fraction collector for capillary electrophoresis
19. Application of constant denaturant capillary electrophoresis (CDCE) to mutation detection in humans
20. Recursion relation generation of probability profiles for specific-sequence macromolecules with long-range correlations
21. DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection
22. Potential use of gradient denaturing gel electrophoresis in obtaining mutational spectra from human cells