The noncompetitive inhibitor WW781 senses changes in erythrocyte anion exchanger (AE1) transport site conformation and substrate binding

Journal of General Physiology

Volumn 115, Issue 2, 2000, Pages 159-173

  Knauf, Philip A ^a,b   Raha, Nancy Mendoza ^a   Spinelli, Laurie J ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Band 3; Obligatory exchange; Oxonol; Ping pong mechanism; Red blood cell

Indexed keywords

5 (1,3 DIBUTYLBARBITURIC ACID) 1 (4 SULFOPHENYL) 3 METHYL 5 PYRAZOLONEPENTAMETHINOXONOL; [3 METHYL 1 [4 SULFOPHENYL 5 PYRAZOLONE (4)]] [1,3 DIBUTYLBARBITURIC ACID]PENTAMETHINE OXONOL; ALCOHOL DERIVATIVE; DYE; ERYTHROCYTE BAND 3 PROTEIN; UNCLASSIFIED DRUG;

ANION EXCHANGE; ARTICLE; BINDING AFFINITY; CHLORIDE CURRENT; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; ERYTHROCYTE; HUMAN; HUMAN TISSUE; KINETICS; NORMAL HUMAN; PROTEIN CONFORMATION; RECEPTOR BINDING;

ANION EXCHANGE PROTEIN 1, ERYTHROCYTE; ANTIPORTERS; BARBITURATES; BINDING SITES; COLORING AGENTS; ERYTHROCYTE MEMBRANE; HUMANS; ISOXAZOLES; KINETICS; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0033818056     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.115.2.159     Document Type: Article

References (34)

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13. Anion transport proteins
14. Cellular anion transport
15. Rapid electrogenic sulfate-chloride exchange mediated by chemically modified band 3 in human erythrocytes
16. 2DIDS
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18. Kinetics of anion transport
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20. The relationship between anion exchange and net anion flow across the human red blood cell membrane
21. An oxonol dye is the most potent known inhibitor of band 3-mediated anion exchange
22. Location of the chloride self-inhibitory site of the human erythrocyte anion exchange system
23. The conformation of the human red blood cell anion exchange protein, band 3, strongly affects binding of the oxonol dye WW781
24. Mechanisms of substrate binding, inhibitor binding, and ion translocation in band 3 and band 3-related proteins
25. DNDS (4,4'-dinitro-stilbene-2,2'-disulfonate) does not act as a purely competitive inhibitor of red blood cell band 3-mediated anion exchange
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27. - affinity
28. - exchange by a 2-step mechanism which is sensitive to substrate binding and transport site reorientation
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31. - transport site of human red blood cells by a kinetic analysis of the inhibitory effects of a chemical probe
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34. Three-dimensional map of the dimeric membrane domain of the human erythrocyte anion exchanger, band 3