Analysis of mutants of tetanus toxin H(C) fragment: Ganglioside binding, cell binding and retrograde axonal transport properties

Molecular Microbiology

Volumn 37, Issue 5, 2000, Pages 1041-1051

  Sinha, Katharine ^a   Box, Michael ^a   Lalli, Giovanna ^b   Schiavo, Giampietro ^b   Schneider, Holm ^c   Groves, Michael ^d   Siligardi, Giuliano ^e   Fairweather, Neil ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

^c IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

^d UNIVERSITY COLLEGE LONDON   (United Kingdom)

^e KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GANGLIOSIDE; TETANUS TOXIN;

ARTICLE; CELL ADHESION; CLOSTRIDIUM TETANI; INTERNALIZATION; INTRACELLULAR TRANSPORT; NERVE FIBER TRANSPORT; NEUROTRANSMITTER RELEASE; NONHUMAN; PRIORITY JOURNAL; TETANUS;

ANIMALS; AXONAL TRANSPORT; CATTLE; CELLS, CULTURED; GANGLIOSIDES; HISTIDINE; MICE; MOTOR NEURONS; MUTAGENESIS; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, SECONDARY; RATS; TETANUS TOXIN;

EID: 0033812830     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.02091.x     Document Type: Article

References (42)

1. Cardiotrophin-1 requires LIFRbeta to promote survival of mouse motoneurons purified by a novel technique
2. An antigenic polypeptide fragment isolated from tetanus toxin: Chemical characterization, binding to gangliosides and retrograde axonal transport in various neuron systems
3. Interaction of 1251-labeled botulinum neurotoxins with nerve terminals. II. Autoradiographic evidence for its uptake into motor nerves by acceptor-mediated endocytosis
4. Interaction of 1251-labelled botulinum neurotoxins with nerve terminals. I. Ultrastructural autoradiographic localization and quantitation of distinct membrane acceptors for types A and B on motor nerves
5. A monoclonal antibody modulates the interaction of nerve growth factor with PC12 cells
6. Construction of hybrid proteins that migrate retrogradely and transynaptically into the central nervous system
7. The structures of the H (C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding
8. Cloning, nucleotide sequencing, and expression of tetanus toxin fragment C in Escherichia coli
9. Characterization of recombinant tetanus toxin derivatives suitable for vaccine development
10. Delivery of recombinant tetanus-superoxide dismutase proteins to central nervous system neurons by retrograde axonal transport
11. Retrograde transneuronal transfer of the C-fragment of tetanus toxin
12. Characterization of fragment C and tetanus toxin binding to rat brain membranes
13. Characterization of the receptor-binding domain of tetanus toxin
14. Neurospecific binding, internalization, and retrograde axonal transport
15. Cloning and expression of functional fragment C of tetanus toxin
16. Pathophysiological properties of clostridial neurotoxins
17. Tetanus toxin fragment C binds to a protein present in neuronal cell lines and motoneurons
18. C-terminal half of tetanus toxin fragment C is sufficient for neuronal binding and interaction with a putative protein receptor
19. The fixation of tetanus toxin by gangliosides
20. DNA vaccines with single-chain Fv fused to fragment C of tetanus toxin induce protective immunity against lymphoma and myeloma
21. Non-viral neuronal gene delivery mediated by the HC fragment of tetanus toxin
22. Sequence homology and structural analysis of the clostridial neurotoxins
23. Crystal structure of botulinum neurotoxin type A and implications for toxicity
24. Functional characterisation of tetanus and botulinum neurotoxins binding domains
25. Isolation of synaptotagmin as a receptor for type A and type E botulinum neurotoxin and analysis of their comparative binding using a new microtitre plate assay
26. Expression of tetanus toxin fragment C in E. coli: High level expression by removing rare codons
27. Gangliosides mediate inhibitory effects of tetanus and botulinum A neurotoxins on exocytosis in chromaffin cells
28. Synaptic vesicle endocytosis mediates the entry of tetanus neurotoxin into hippocampal neurons
29. Molecular genetics of clostridial neurotoxins
30. How do tetanus and botulinum neurotoxins bind to neuronal membranes?
31. Molecular Biology of Clostridial Neurotoxins
32. Identification of protein receptor for Clostridium botulinum type B neurotoxin in rat brain synaptosomes
33. Manipulation of pathogen-derived genes to influence antigen presentation via DNA vaccines
34. Identification of a ganglioside recognition domain of tetanus toxin using a novel ganglioside photoaffinity ligand
35. Molecular pharmacology of botulinum and tetanus toxin
36. Characterization of the binding and internalization of tetanus toxin in a neuroblastoma hybrid cell line
37. Structure of the receptor binding fragment HC of tetanus neurotoxin
38. Bafilomycin A1 inhibits the action of tetanus toxin in spinal cord neurons in cell culture
39. Neuronal sensitivity to tetanus toxin requires gangliosides
40. An immunohistochemical study of the nerve growth factor receptor in developing rats