Immobilizing the moving parts of voltage-gated ion channels

Journal of General Physiology

Volumn 116, Issue 3, 2000, Pages 461-475

  Horn, Richard ^a   Ding, Shinghua ^a   Gruber, Hermann J ^b  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b JOHANNES KEPLER UNIVERSITY LINZ   (Austria)

Author keywords

Benzophenone; Cysteine mutagenesis; S4 segment; Shaker potassium channel; Sodium channel

Indexed keywords

BENZOPHENONE; CYSTEINE; ION CHANNEL; POTASSIUM CHANNEL; POTASSIUM ION; SODIUM CHANNEL; SODIUM ION;

ARTICLE; CROSS LINKING; GENE MUTATION; HUMAN; HUMAN TISSUE; SKELETAL MUSCLE; ULTRAVIOLET IRRADIATION;

BASE SEQUENCE; CELL LINE; CROSS-LINKING REAGENTS; DNA PRIMERS; HUMANS; ION CHANNEL GATING; MUSCLE, SKELETAL; MUTAGENESIS, SITE-DIRECTED; POTASSIUM CHANNELS; RECOMBINANT PROTEINS; SHAKER SUPERFAMILY OF POTASSIUM CHANNELS; SODIUM CHANNELS;

EID: 0033811116     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.116.3.461     Document Type: Article

References (67)

1. Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axons
2. Inactivation of the sodium channel. II. Gating current experiments
3. + channel gating
4. The voltage sensor in voltage dependent ion channels
5. Inactivation of the sodium channel. I. Sodium current experiments
6. + channels: II. The components of gating currents and a model of channel activation
7. Molecular motions within the pore of voltage-dependent sodium channels
8. Molecular properties of voltage-sensitive sodium channels
9. Voltage sensor-trapping: Enhanced activation of sodium channels by β-scorpion toxin bound to the S3-S4 loop in domain II
10. + channel with fluorescence
11. + channel
12. + channel fast inactivation
13. Atomic scale movement of the voltage-sensing region in a potassium channel measured via spectroscopy
14. Sodium channel mutations in paramyotonia congenita uncouple inactivation from activation
15. A unique role for the S4 segment of domain 4 in the inactivation of sodium channels
16. + channel and its structural implications
17. Cysteine scanning analysis of the IFM cluster in the inactivation gate of a human heart sodium channel
18. Benzophenone photophores in biochemistry
19. Point mutations in IIS4 alter activation and inactivation of rat brain IIA Na channels in Xenopus oocyte macropatches
20. Protein structural dynamics by single molecule fluorescence polarization
21. Interactions between a pore-blocking peptide and the voltage sensor of a sodium channel: An electrostatic approach to channel geometry
22. Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel
23. Inactivation of the sodium current in Myxicola giant axons: Evidence for coupling to the activation process
24. Gating of Na channels. Inactivation modifiers discriminate among models
25. Differential effects of homologous S4 mutations in human skeletal muscle sodium channels on deactivation gating from open and inactivated states
26. + channel can be trapped in the open state by an intersubunit metal bridge
27. + channels: Evidence for a trap door mechanism of activation gating
28. Monitoring conformational changes with site directed spin labeling
29. Paramyotonia congenita mutations reveal different roles for segments S3 and S4 of domain D4 in hSkM1 sodium channel gating
30. + channel inactivation gate during inactivation
31. The kinetics of voltage-gated ion channels
32. The screw-helical voltage gating of ion channels
33. Sodium channel inactivation is altered by substitution of voltage sensor positive charges
34. Sodium channel activation gating is affected by substitutions of voltage sensor positive charges in all four domains
35. Mutations in the S4 region isolate the final voltage-dependent cooperative step in potassium channel activation
36. + channel
37. + channel during gating
38. Independence and cooperativity in rearrangements of a potassium channel voltage sensor revealed by single subunit fluorescence
39. Direct physical measure of conformational rearrangement underlying potassium channel gating
40. + solutions: Role of the voltage sensor
41. Independent versus coupled inactivation in sodium channels: Role of the domain 2 S4 segment
42. Role of domain 4 in sodium channel slow inactivation
43. A piston model for transmembrane signaling of the aspartate receptor
44. +-channel inactivation: Charge neutralizations and deletions in the III-IV linker
45. +-channel activation gating
46. Solution structure of the sodium channel inactivation gate
47. Activation of Shaker potassium channels III. An activation gating model for wild-type and V2 mutant channels
48. Voltage-dependent open-state inactivation of cardiac sodium channels: Gating current studies with Anthopleurin-A toxin
49. The role of the putative inactivation lid in sodium channel gating current immobilization
50. The Na channel voltage sensor associated with inactivation is localized to the external charged residues of domain IV, S4
51. Voltage gating of ion channels
52. Identification of a translocated protein segment in a voltage-dependent channel
53. Role of the S4 in cooperativity of voltage-dependent potassium channel activation
54. Uncharged S4 residues and cooperativity in voltage-dependent potassium channel activation
55. Protein Motions
56. + channel through multiple binding sites
57. + channels
58. + channel pores revealed by single- and double-cysteine replacements
59. Evidence for cooperative interactions in potassium channel gating
60. The way things move: Looking under the hood of molecular motor proteins
61. Identification of an intracellular peptide segment involved in sodium channel inactivation
62. Fluorescence spectroscopy of single biomolecules
63. +-channel inactivation
64. Molecular basis of charge movement in voltage-gated sodium channels
65. Evidence for voltage-dependent S4 movement in sodium channels
66. The moving parts of voltage-gated ion channels
67. Dynamic transitions of the transmembrane domain of diphtheria toxin: Disulfide trapping and fluorescence proximity studies