Cloning and heterologous expression of Dα4, a Drosophila neuronal nicotinic acetylcholine receptor subunit: Identification of an alternative exon influencing the efficiency of subunit assembly

Neuropharmacology

Volumn 39, Issue 13, 2000, Pages 2604-2614

  Lansdell, Stuart J ^a   Millar, Neil S ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Alternative splicing; Nicotinic acetylcholine receptor; Subunit assembly

Indexed keywords

COMPLEMENTARY DNA; EPIBATIDINE; NICOTINIC RECEPTOR; RECEPTOR SUBUNIT;

ALTERNATIVE RNA SPLICING; ANIMAL CELL; ARTICLE; CLONING; DROSOPHILA MELANOGASTER; EXON; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMAL; BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE; CHROMOSOMES; DROSOPHILA; EXONS; INTRONS; MOLECULAR SEQUENCE DATA; NEURONS; PLASMIDS; RADIOLIGAND ASSAY; RECEPTORS, NICOTINIC; SUPPORT, NON-U.S. GOV'T;

EID: 0033809872     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(00)00111-8     Document Type: Article

References (42)

1. The genome sequence of Drosophila melanogaster
2. Sequence of Dα2, a novel α-like subunit of Drosophila nicotinic acetylcholine receptors
3. Physiological properties of neuronal nicotinic receptors reconstituted from the vertebrate β2 subunit and Drosophila α subunits
4. Mutational analysis of muscle nicotinic acetylcholine receptor subunit assembly
5. Conservation of neural nicotinic acetylcholine receptors from Drosophila to vertebrate central nervous systems
6. Molecular properties and functions of insect acetylcholine receptors
7. Characterization and use of the Drosophila metallothionein promoter in cultured Drosophila melanogaster cells
8. Identification of amino acids contributing to high and low affinity d-tubocurarine sites in the Torpedo nicotinic acetylcholine receptor
9. 3H]nicotine photoincorporation in the γ-subunit of the Torpedo nicotinic acetylcholine receptor
10. Upregulation of cell-surface α4β2 neuronal nicotinic receptors by lower temperature and expression of chimeric subunits
11. Host cell-specific folding and assembly of the neuronal nicotinic acetylcholine receptor α7 subunit
12. Pharmacological and functional diversity of neuronal nicotinic acetylcholine receptors
13. Characterization of a nicotinic acetylcholine receptor from the insect Manduca sexta
14. Asymmetric contribution of the conserved disulphide loop to subunit oligomerization and assembly of the nicotinic acetylcholine receptor
15. Genomic organization and partial duplication of the human α7 neuronal nicotinic acerylcholine receptor gene (CHRNA7)
16. Ion-channel assembly
17. The role of the cystine loop in acetylcholine receptor assembly
18. Primary structure of a developmentally regulated nicotinic acetylcholine receptor protein from Drosophila
19. Neuronal nicotinic receptors in the Locust Locusta migratoria: cloning and expression
20. A short polypeptide marker sequence useful for recombinant protein identification and purification
21. Molecular characterization and imidacloprid selectivity of nicotinic acetylcholine receptor subunits from the peach-potato aphid Myzus persicae
22. Cloning, heterologous expression and co-assembly of Mpβ1, a nicotinic acetylcholine receptor subunit from the aphid Myzus persicae
23. A high throughput screen to identify secreted and transmembrane proteins involved in Drosophila embryogenesis
24. The influence of nicotinic receptor subunit composition upon agonist, α-bungarotoxin and insecticide (imidacloprid) binding affinity
25. Temperature-sensitive expression of Drosophila neuronal nicotinic acetylcholine receptors
26. Imidacloprid - a chloronicotinyl insecticide: biological activity and agricultural significance
27. Sequence and functional expression of a single α subunit of an insect nicotinic acetylcholine receptor
28. Physiological diversity of nicotinic acetylcholine receptors expressed by vertebrate neurons
29. Heterologous expression of mammalian and insect neuronal nicotinic acetylcholine receptors in cultured cell lines
30. Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line
31. Neuronal ion channels as the target sites of insecticides
32. Primary structure of α-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequence
33. Evolutionary history of the ligand-gated ion-channel superfamily of receptors
34. Acetylcholine receptors of insects
35. Heterogeneity of Drosophila nicotinic acetylcholine receptors: SAD, a novel developmentally regulated α-subunit
36. SBD, a novel structural subunit of the Drosophila nicotinic acetylcholine receptor, shares its genomic localization with two α-subunits
37. Cell lines derived from late embryonic stages of Drosophila melanogaster
38. Dα3, a new functional α subunit of nicotinic acetylcholine receptors from Drosophila
39. Cloning and functional characterisation of two novel nicotinic acetylcholine receptor α subunits from the insect pest Myzus persicae
40. Assembly of mutant subunits of the nicotinic acetylcholine receptor lacking the conserved disulfide loop structure
41. Projection structure of nicotinic acetylcholine receptor: distinct conformations of the α subunits
42. A new method for predicting signal sequence cleavage sites