Overproduction and improved strategies to purify the three native forms of nuclease-free HU protein

Biochimie

Volumn 82, Issue 8, 2000, Pages 693-704

  Pellegrini, Olivier ^a   Oberto, Jacques ^a   Pinson, Valérie ^a   Wery, Muriel ^a   Rouviere Yaniv, Josette ^a  

^a INST DE BIOLOGIE PHYSICO CHIMIQUE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; CRUCIFORM DNA; DIMER; DNA BINDING PROTEIN; DOUBLE STRANDED DNA; HIGH MOBILITY GROUP PROTEIN; HISTIDINE; HISTONE; NUCLEASE; NUCLEIC ACID; OLIGONUCLEOTIDE; PROTEIN SUBUNIT;

ANIMAL CELL; ARTICLE; BACTERIUM; CELL DIFFERENTIATION; CELL GROWTH; CHLOROPLAST; DNA REPAIR; DNA SEQUENCE; DNA STRUCTURE; DNA SUPERCOILING; ESCHERICHIA COLI; IN VIVO STUDY; INTERACTIONS WITH NUCLEIC ACID; NONHUMAN; PROTEIN ISOLATION; PROTEIN PURIFICATION; VIRUS;

EID: 0033809250     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-9084(00)01151-2     Document Type: Article

References (24)

1. Characterization of a novel, low-molecular-weight DNA-binding protein from Escherichia coli
2. Escherichia coli DNA-binding protein HU forms nucleosome-like structure with circular double stranded DNA
3. Localization of the HU protein on the Escherichia coli nucleoid
4. Native Escherichia coli HU protein is a heterotypic dimer
5. Variation in HU composition during growth of Escherichia coli: The heterodimer is required for long term survival
6. Cyanobacterial DNA-binding protein related to Escherichia coli HU
7. Similarity between the bacterial histone-like protein HU and a protein from spinach chloroplasts
8. Histones, HMG, HU, IHF: Meme combat
9. Serratia marcescens contains a heterodimeric HU protein like Escherichia coli and Salmonella typhimurium
10. Differential binding of the Escherichia coli HU, homodimeric forms and heterodimeric form to linear, gapped and cruciform DNA
11. The Escherichia coli ribosomal protein S16 is an endonuclease
12. Genetic characterization of the gene hupA encoding the HU-2 protein of Escherichia coli
13. Genetic characterization of the gene hupB encoding the HU-1 protein of Escherichia coli
14. The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription
15. Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies
16. DNA sequencing with chain-terminating inhibitors
17. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system
18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
19. Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter
20. DNA-binding parameters of the HU protein of Escherichia coli to cruciform DNA
21. An imbalance of HU synthesis induces mucoidy in Escherichia coli
22. Multiple defects in Escherichia coli mutants lacking HU protein
23. Inhibition of cell division in hupA hupB mutant bacteria lacking HU protein