The role of Glu74 and Tyr82 in the reaction catalyzed by sheep liver cytosolic serine hydroxymethyltransferase

European Journal of Biochemistry

Volumn 267, Issue 19, 2000, Pages 5967-5976

  Krishna Rao, J V ^a   Prakash, V ^b   Appaji Rao, N ^a   Savithri, H S ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

Active site residues; Conformational change; Proton abstraction; Serine hydroxymethyltransferase; Spectral intermediates

Indexed keywords

ACETAL DERIVATIVE; ALANINE; DIHYDROFOLATE REDUCTASE; FORMALDEHYDE; GLUTATHIONE; GLYCINE HYDROXYMETHYLTRANSFERASE; RECOMBINANT ENZYME; SERINE; THREONINE; TYROSINE;

ARTICLE; CHEMICAL BOND; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; LIVER CYTOSOL; NONHUMAN; PRIORITY JOURNAL; SHEEP; TRANSAMINATION;

ALANINE; AMINO ACID SUBSTITUTION; ANIMALS; APOENZYMES; BINDING SITES; CALORIMETRY, DIFFERENTIAL SCANNING; CATALYSIS; CYTOSOL; FORMALDEHYDE; GLUTAMIC ACID; GLYCINE HYDROXYMETHYLTRANSFERASE; HUMANS; KINETICS; LIVER; MUTAGENESIS, SITE-DIRECTED; PYRIDOXAL PHOSPHATE; RABBITS; RECOMBINANT FUSION PROTEINS; SERINE; SHEEP; STRUCTURE-ACTIVITY RELATIONSHIP; TETRAHYDROFOLATES; TYROSINE;

ORYCTOLAGUS CUNICULUS; OVIS ARIES;

EID: 0033807968     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01667.x     Document Type: Article

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