Mechanisms of the release of CYFRA21-1 in human lung cancer cell lines

Lung Cancer

Volumn 30, Issue 1, 2000, Pages 55-63

  Dohmoto, Kazutaka ^a   Hojo, Satoko ^a   Fujita, Jiro ^a   Ueda, Yutaka ^a   Bandoh, Shuji ^a   Yamaji, Yasufumi ^b   Ohtsuki, Yuji ^c   Dobashi, Naomi ^a   Takahara, Jiro ^a  

^a KAGAWA UNIVERSITY   (Japan)

^b Mitoyo General Hospital   (Japan)

^c KOCHI MEDICAL SCHOOL   (Japan)

Author keywords

CYFRA 21 1; Cytokeratin 19; Lung cancer cell lines; Quantitative PCR

Indexed keywords

CELL DNA; CYTOKERATIN 19 FRAGMENT; MESSENGER RNA;

ARTICLE; CANCER CELL CULTURE; CANCER CYTODIAGNOSIS; CONTROLLED STUDY; HUMAN; HUMAN CELL; LUNG CANCER; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SUPERNATANT;

EID: 0033801790     PISSN: 01695002     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-5002(00)00125-2     Document Type: Article

References (21)

1. Moll R., Franke W.W., Schiller D.L., Geiger B., Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 31:1982;11-24.
2. Steinert P.M., Bale S.J. Genetic skin diseases caused by mutations in keratin intermediate filaments. Trends Genet. 9:1993;280-284.
3. Pujol J.L., Grenier J., Daures J.P., Daver A., Pujol H., Michel F.B. Serum fragment of cytokeratin subunit 19 measured by CYFRA 21-1 immunoradiometric assay as a marker of lung cancer. Cancer Res. 53:1993;61-66.
4. Satoh H., Ishikawa H., Fujiwara M., Yamashita Y.T., Ohtsuka M., Ogata T., Hasegawa S., Kamma H. Production of cytokeratin 19 fragment by human squamous lung cancer cell lines. Am. J. Respir. Cell. Mol. Biol. 16:1997;597-604.
5. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
6. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
7. Sato M., Mizobuchi M., Murao K., Tamaki M., Takahara J. Simple quantitative reverse transcribed-polymerase chain reaction (RT-PCR) method involving recombinant RNA generated by a false-priming PCR product. J. Biochem. 116:1994;1198-1201.
8. Boldrini L., Calcinai A., Silvestri V., Basolo F., Lucchi M., Mussi A., Angeletti C.A., Bevilacqua G., Fontanini G. Quantitation by competitive PCR assay of vascular endothelial growth factor in non-small cell lung carcinomas. Int. J. Oncol. 14:1999;161-168.
9. Savtchenko E.S., Schiff T.A., Jiang C.K., Freedberg I.M., Blumenberg M. Embryonic expression of the human 40-kD keratin: evidence from a processed pseudogene sequence. Am. J. Hum. Genet. 43:1988;630-637.
10. Ruud P., Fodstad O., Hovig E. Identification of a novel cytokeratin 19 pseudogene that may interfere with reverse transcriptase-polymerase chain reaction assays used to detect micrometastatic tumor cells. Int. J. Cancer. 80:1999;119-125.
11. Datta Y.H., Adams P.T., Drobyski W.R., Ethier S.P., Terry V.H., Roth M.S. Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction. J. Clin. Oncol. 12:1994;475-482.
12. Bostick P.J., Chatterjee S., Chi D.D., Huynh K.T., Giuliano A.E., Cote R., Hoon D.S. Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients. J. Clin. Oncol. 16:1998;2632-2640.
13. Schoenfeld A., Luqmani Y., Smith D., O'Reilly S., Shousha S., Sinnett H.D., Coombes R.C. Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction. Cancer Res. 54:1994;2986-2990.
14. Ueda Y., Fujita J., Bandoh S., Hojo S., Yamaji Y., Ohtsuki Y., Dobashi N., Takahara J. Expression of cytokeratin 19 mRNA in human lung cancer cell lines. Int. J. Cancer. 81:1999;939-943.
15. Bodenmuller H., Ofenloch-Hahnle B., Lane E.B., Dessauer A., Bottger V., Donie F. Lung cancer-associated keratin 19 fragments: development and biochemical characterization of the new serum assay Enzymun-Test CYFRA 21-1. Int. J. Biol. Markers. 9:1994;75-81.
16. Pujol J.L., Grenier J., Parrat E., Lehmann M., Lafontaine T., Quantin X., Michel F.B. Cytokeratins as serum markers in lung cancer: a comparison of CYFRA 21-1 and TPS. Am. J. Respir. Crit. Care Med. 154:1996;725-733.
17. Ku N.O., Liao J., Omary M.B. Apoptosis generates stable fragments of human type I keratins. J. Biol. Chem. 272:1997;33197-33203.
18. Brabon A.C., Williams J.F., Cardiff R.D. A monoclonal antibody to a human breast tumor protein released in response to estrogen. Cancer Res. 44:1984;2704-2710.
19. Hu L., Gudas L.J. Activation of keratin 19 gene expression by a 3′ enhancer containing an AP1 site. J. Biol. Chem. 269:1994;183-191.
20. Paone G., De Angelis G., Munno R., Pallotta G., Bigioni D., Saltini C., Bisetti A., Ameglio F. Discriminant analysis on small cell lung cancer and non-small cell lung cancer by means of NSE and CYFRA-21.1. Eur. Respir. J. 8:1995;1136-1140.
21. Burchill S.A., Bradbury M.F., Pittman K., Southgate J., Smith B., Selby P. Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Br. J. Cancer. 71:1995;278-281.