A fast and efficient method for transiently transfecting ES cells: Application to the development of systems for conditional gene expression

Transgenic Research

Volumn 9, Issue 3, 2000, Pages 229-232

  Bugeon, L ^a   Syed, N ^a   Dallman, M J ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Conditional gene expression; Ecdysone; Embryonic stem cells; Gene targeting; Liposome; Transfection; Transgenic mouse

Indexed keywords

GENE EXPRESSION REGULATION; LIPOSOME; MOUSE; PROCESS OPTIMIZATION; STEM CELL TRANSPLANTATION;

CELLS, CULTURED; GENE EXPRESSION; GENETIC SCREENING; IMMUNOENZYME TECHNIQUES; INTEGRASE; LAC OPERON; LIPOSOMES; SENSITIVITY AND SPECIFICITY; STEM CELLS; SUPPORT, NON-U.S. GOV'T; TRANSFECTION;

EID: 0033801724     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008990510849     Document Type: Article

References (4)

1. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters
2. Efficient selection for high-expression transfectants with a novel eukaryotic vector
3. Ecdysone-inducible gene expression in mammalian ceils and transgenic mice
4. Non-injection methods for the production of embryonic stem cell-embryo chimaeras