Microassay for rapid screening of α-amylase activity

Journal of Agricultural and Food Chemistry

Volumn 48, Issue 10, 2000, Pages 4540-4543

  Wong, D W S ^a   Batt, S B ^a   Robertson, G H ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Amylase; Barley amylase; Microassay

Indexed keywords

AMYLASE; DYE; ISOENZYME; RECOMBINANT PROTEIN; STARCH;

ARTICLE; BARLEY; CHEMISTRY; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; YEAST CELL;

ALPHA-AMYLASE; COLORING AGENTS; ISOENZYMES; MICROCHEMISTRY; RECOMBINANT PROTEINS; STARCH;

HORDEUM VULGARE SUBSP. VULGARE;

EID: 0033792288     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf000043z     Document Type: Article

References (11)

1. Laboratory methods for alpha-amylase analyses
2. Determination of cereal alpha amylase using a commercially available dye-labeled substrate
3. A new method for determination of α-amylase
4. Miniaturization of three carbohydrate analyses using a microsample plate reader
5. Expression and secretion of rice α-amylase by Saccharomyces cerevisiae
6. Starch conversion by soluble and immobilized α-amylase
7. Determination of specific activities of malt α-amylases
8. Detection of sprouted wheat by a rapid colorimetric determination of α-amylase
9. A new method for the determination of α-amylase
10. Expression of cDNAs encoding barley α-amylase 1 and 2 in yeast and characterization of the secreted proteins
11. Assay of reducing sugars in the nanomole range with 2,2'-bicinchoninate