Continuous production and recovery of recombinant Ca2+ binding receptor from HEK 293 cells using perfusion through a packed bed bioreactor

Cytotechnology

Volumn 33, Issue 1-3, 2000, Pages 3-11

  Kaufman, J B ^a   Wang, G ^b   Zhang, W ^b   Valle, M A ^a   Shiloach, J ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b New Brunswick Scientific   (United States)

Author keywords

HEK 293; Packed bed bioreactor; Perfusion

Indexed keywords

CALCIUM RECEPTOR; CELL LINE; CULTURE MEDIUM; GLUCOSE; HUMAN CELL; KIDNEY CELL; PACKED BED REACTOR; PARATHYROID GLAND; PERFUSION; PROCESS OPTIMIZATION;

EID: 0033791103     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008143132056     Document Type: Conference Paper

References (13)

1. High level expression of secreted protein from cells adapted to serum-free suspension culture
2. A fiber-bed bioreactor for anchorage-dependent animal cell cultures: Part I. Bioreactor design and operation
3. The development of large-scale animal cell perfusion culture system
4. Expression, purification, and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor
5. Growth of 293 in suspension culture
6. Characteristics of a human cell line transformed by DNA from human adenovirus type 5
7. Packed bed bioreactor with porous ceramic beads for animal cell culture
8. Investigation of parameters affecting a fixed bed bioreactor process for recombinant cell lines
9. Culture of 293 cells in different culture systems: Cell growth and recombinant adenovirus production
10. The application of a new type of sintered glass carriers for the cultivation of anchorage-dependent mammalian cells
11. Cell culture bioreactors
12. 2+ receptor from HEK 293 cells using novel serum free medium
13. Scale up of interluekin-6 production by BHK cells using a radical-flow reactor packed with porous glass beads