Use of an IRES bicistronic construct to trace expression of exogenously introduced mRNA in zebrafish embryos

BioTechniques

Volumn 29, Issue 4, 2000, Pages 814-820

  Wang, X ^a   Wan, H ^a   Korzh, V ^a   Gong, Z ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; PROTEIN ISLET 1; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CISTRON; EMBRYO; GENE CONSTRUCT; GENE EXPRESSION; GENE FUNCTION; GENETIC ANALYSIS; NONHUMAN; PLASMID; REPORTER GENE; RIBOSOME; RNA TRANSLATION; ZEBRA FISH;

ANIMALIA; DANIO RERIO; VERTEBRATA;

EID: 0033786440     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00294st09     Document Type: Article

References (11)

1. Green flurescent protein as a marker for gene expression
2. pIRES-CD4t, a dicistronic expression vector for MACS or FACS-based selection of transfected cells
3. The internal ribosomal entry site of the encephalomyelitis virus enables reliable coexpression of two transgenes in human primary T lymphocytes
4. The hepatitis C virus internal ribosome entry site adopts and ion-dependent tertiary fold
5. Expression of zebrafish bHLH genes ngn1 and nrd defines distinct stages of neural differentiation
6. Conversion of Xenopus ectoderm into neurons by Neuro-D, a basic helix-loop-helix protein
7. A class of NeuroD-related basic helix-loop-helix transcription factors expressed in developing central nervous system in zebrafish
8. Internal ribosome entry site biology and its use in expression vectors
9. Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis
10. Xenopus embryos regulate the nuclear localization of xMyoD
11. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells