Mutation of the ATP-binding pocket of SSA1 indicates that a functional interaction between Ssa1p and Ydj1p is required for post-translational translocation into the yeast endoplasmic reticulum

Genetics

Volumn 156, Issue 2, 2000, Pages 501-512

  McClellan, Amie J ^a   Brodsky, Jeffrey L ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; FUNGAL PROTEIN; HEAT SHOCK PROTEIN 70; MUTANT PROTEIN; PROTEIN DNAK; SSA1 PROTEIN; SSA1P PROTEIN; UNCLASSIFIED DRUG; YDJ1P PROTEIN;

ALLELE; ARTICLE; CELL GROWTH; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; MOLECULAR CLONING; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN TRANSPORT; SACCHAROMYCES CEREVISIAE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; AMINO ACID SUBSTITUTION; BINDING SITES; ENDOPLASMIC RETICULUM; FUNGAL PROTEINS; GENOTYPE; HEAT-SHOCK PROTEINS; HSP40 HEAT-SHOCK PROTEINS; HSP70 HEAT-SHOCK PROTEINS; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0033785942     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (54)

1. Real time kinetics of the DnaK/DnaJ/GrpE molecular chaperone machine action
2. Functional interaction of cytosolic hsp70 and a DnaJ-related protein, Ydj1p, in protein translocation in vivo
3. Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic hsc70
4. Sec63p and BiP are required for both co-and post-translational protein translocation into yeast microsomes
5. The hsp70 and hsp60 chaperone machines
6. Secretion in yeast: Purification and in vitro translocation of chemical amounts of prepro-alpha-factor
7. YDJ1p facilitates polypeptide translocation across different intracellular membranes by a conserved mechanism
8. 70K Heat shock related proteins stimulate protein translocation into microsomes
9. Conformational changes of an hsp70 molecular chaperone induced by nucleotides, polypeptides, and N-ethylmaleimide
10. Mutations in cognate genes of Saccharomyces cerevisiae hsp70 result in reduced growth rates at low temperatures
11. The folding of the bifuncfional TRP3 protein in yeast is influenced by a translational pause which lies in a region of structural divergence with Eschericia coli indoleglycerol-phosphate synthase
12. Regulation of hsp70 function by a eukaryotic DnaJ homolog
13. Intragenic suppressors of Hsp70 mutants: Interplay between the ATPase- and peptide-binding domains
14. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor proteins
15. Peptide binding and release by proteins implicated as catalysts of protein assembly
16. Common and divergent peptide binding specificities of hsp70 molecular chaperones
17. Identification of a regulatory motif in hsp70 that affects ATPase activity, substrate binding and interaction with HDJ-1
18. Conformations of the nucleotide and polypeptide binding domains of cytosolic hsp70 molecular chaperones are coupled
19. Mutations in the DnaK chaperone affecting interaction with the DnaJ cochaperone
20. Role of the J-domain in the cooperation of Hsp40 with Hsp70
21. Molecular chaperones in cellular protein folding
22. N-ethylmaleimide-modified hsp70 inhibits protein folding
23. The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import
24. Yeast/E. coli shuttle vectors with multiple unique restriction sites
25. Import into and degradation of cytosolic proteins by isolated yeast vacuoles
26. The translocon: A dynamic gateway at the ER membrane
27. Folding in vivo of a newly translated yeast cytosolic enzyme is mediated by the SSA class of cytosolic yeast hsp70 proteins
28. Copper ion inducible and repressible promoter systems in yeast
29. N-ethylmaleimide inactivates a nucleotide-free hsp70 molecular chaperone
30. Protein folding activity of hsp70 is modified differentially by the hsp40 co-chaperones Sis1 and Ydj1
31. The role of ATP in the functional cycle of the DnaK chaperone system
32. Specific molecular chaperone interactions and an ATP-dependent conformadonal change are required during post-translational protein translocatlon into the yeast ER
33. Mge1 functions as a nucleotide release factor for Ssc1, a mitochondrial hsp70 of Saccharomyces cerevisiae
34. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds
35. Isolation and characterization of extragenic suppressors of mutations in the SSA hsp70 genes of Saccharomyces cerevisiae
36. Functional analysis of a conserved amino-terminal region of hsp70 by site-directed mutagenesis
37. Loss of hsp70-hsp40 chaperone activity causes abnormal nuclear distribution and aberrant microtubule formation in M-phase of Saccharomyces cerevisiae
38. Interaction of hsp70 with unfolded proteins: Effects of temperature and nucleotides on the kinetics of binding
39. The molecular chaperone Ssb from Saccharomyces cerevisiae is a component of the ribosome-nascent chain complex
40. Characterization of D10S and K71E mutants of human cytosolic hsp70
41. Molecular chaperones: Towards a characterization of the heat-shock protein 70 family
42. Sec61p and BiP directly facilitate polypeptide translocation into the ER
43. Kinetics of molecular chaperone action
44. In vivo nuclear transport kinetics in Saccharomyces cerevisiae: A role for heat shock protein 70 during targeting and translocation
45. In vitro mutagenesis and plasmid shuffling: From cloned gene to mutant yeast
46. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
47. The amino-terminal transforming region of simian virus 40 large T and small t antigens functions as a J domain
48. Interaction of the Hsp70 molecular chaperone, DnaK, with its cochaperone DnaJ
49. Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis
50. In vitro dissociation of BiP-peptide complexes requires a conformational change in BiP after ATP binding but does not require ATP hydrolysis
51. Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae
52. The dissociation of ATP from hsp70 of Saccharomyces cerevisiae is stimulated by both Ydj1p and peptide substrates