Development of a vital fluorescent staining method for monitoring bacterial transport in subsurface environments

Applied and Environmental Microbiology

Volumn 66, Issue 10, 2000, Pages 4486-4496

  Fuller, M E ^a   Streger, S H ^a   Rothmel, R K ^a   Mailloux, B J ^a   Hall, J A ^a   Onstott, T C ^a   Fredrickson, J K ^a   Balkwill, D L ^a   Deflaun, M F ^a  

^a The Shaw Group Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

6 CARBOXYFLUORESCEIN; GROUND WATER; SUCCINIMIDE DERIVATIVE;

BACTERIUM; FLUORESCENCE; MONITORING; SUBSURFACE FLOW; TRANSPORT PROCESS;

ACCURACY; ARTICLE; BACTERIAL TRANSLOCATION; BACTERIUM DETECTION; CELL ADHESION; CELL CULTURE; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; ENVIRONMENTAL MONITORING; FLOW CYTOMETRY; MICROSCOPY; NONHUMAN; SEDIMENT; SPECTROFLUOROMETRY; STAINING;

COMAMONAS; FLUORESCEINS; FLUORESCENT DYES; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; MICROSCOPY, FLUORESCENCE; SUCCINIMIDES; SUPPORT, U.S. GOV'T, NON-P.H.S.; WATER MICROBIOLOGY;

COMAMONAS;

EID: 0033779656     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.10.4486-4496.2000     Document Type: Article

References (55)

1. Virus and bacteria transport in a sandy aquifer, Cape Cod, MA
2. Application of tetrazolium dye as an indicator of viability in anaerobic bacteria
3. A simple method for counting small numbers of cells by flow cytometry
4. A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester
5. Rapid fluorescence assessment of the viability of stressed Lactococcus lactis
6. Bacterial transport in fractured rock - A field-scale tracer test at the Chalk River Nuclear Laboratories
7. Preliminary observations on bacterial transport in a coastal plain aquifer
8. Alterations in adhesion, transport, and membrane characteristics in an adhesion-deficient pseudomonad
9. Development of an adhesion assay and characterization of an adhesion-deficient mutant of Pseudomonas fluorescens
10. Pilot-scale evaluation of bioaugmentation for in situ remediation of a carbon tetrachloride-contaminated aquifer
11. Applications of the green fluorescent protein as a molecular marker in environmental microorganisms
12. Green fluorescent protein as a marker to monitor survival of phenanthrene-mineralizing Pseudomonas sp. UG14Gr in creosote-containing soil
13. Direct and rapid analysis of the adhesion of bacteria to solid surfaces: Interaction of fiuorescently labeled Rhodococcus strain GIN-1 (NCIMB 40340) cells with titanium-rich particles
14. Examining bacterial transport in intact cores from oyster, Virginia: Effect of sedimentary facies type on bacterial breakthrough and retention
15. Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans
16. In situ and laboratory methods to study subsurface microbial transport
17. Use of colloid filtration theory in modeling movement of bacteria through a contaminated sandy aquifer
18. Transport of microspheres and indigenous bacteria through a sandy aquifer: Results of natural- and forced-gradient tracer experiments
19. Role of physical heterogeneity in the interpretation of small-scale laboratory and field observations of bacteria, microbial-sized microspheres, and bromide transport through aquifer sediments
20. Effect of aerosolization on culturability and viability of gram-negative bacteria
21. Ferrographic tracking of bacterial transport in the field at Oyster, VA
22. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: Past and present
23. Validity of fluorochrome-stained bacteria as tracers of short-term microbial transport through porous media
24. Survival and activity of Pseudomonas sp. strain B13(FR1) in a marine microcosm determined by quantitative PCR and an rRNA-targeting probe and its effect on the indigenous bacterioplankton
25. Effect of growth conditions and surface characteristics of aquatic bacteria on their attachment to solid surfaces
26. Analysis of in vitro lymphocyte adhesion and transendothelial migration by fluorescent-beads-based flow cytometric cell counting
27. In situ biodegradation of TCE contaminated groundwater
28. Green fluorescent protein-marked Pseudomonas fluorescens: Localization, viability, and activity in the natural barley rhizosphere
29. Formation of viable but nonculturable cells
30. Entry into, and resuscitation from, the viable but nonculturable state by Vibrio vulnificus in an estuarine environment
31. Fluorescent dyes for lymphocyte migration and proliferation studies
32. The effect of the minor groove binding agent DAPI (2-aminodiphenyl-indole) on DNA-directed enzymes: An attempt to explain inhibition of plasmid expression in Escherichia coli
33. Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlight
34. Factors affecting the determination of respiratory activity on the basis of cyanoditolyl tetrazolium chloride reduction with membrane filtration
35. Methionine uptake and cytopathogenicity of viable but non-culturable Shigella dysenteriae type 1
36. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria
37. Survival strategies of bacteria in the natural environment
38. Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water
39. Comparison between model simulations and field results for in-situ biorestoration of chlorinated aliphatics: Part 2. Cometabolic transformations
40. Effects of substrates and phosphate on INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride) and CTC (5-cyano-2,3-ditolyl tetrazolium chloride) reduction in Escherichia coli
41. Field-scale evaluation of in situ bioaugmentation for remediation of chlorinated solvents in groundwater
42. Quantitation of cell concentration using the flow cytometer
43. Use of molecular techniques to evaluate the survival of a microorganism injected into an aquifer
44. Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction
45. A new fluorescent staining assay for visualizing living microorganisms in soil
46. Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury
47. Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride
48. Quantitative detection of Sphingomonas chlorophenolica in soil via competitive polymerase chain reaction
49. Survival of, and induced stress resistance in, carbon-starved Pseudomonas fluorescens cells residing in soil
50. Population dynamics of phenol-degrading bacteria in activated sludge determined by gyrB-targeted quantitative PCR
51. Viability of indigenous soil bacteria assayed by respiratory activity and growth
52. Tracking the response of Burkholderia cepacia G4 5223-PR1 in aquifer microcosms
53. Rapid in situ assessment of physiological activities in bacterial biofilms using fluorescent probes
54. Optimal staining and sample storage time for direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes