Expression of a gene encoding the major antigenic protein 2 homolog of Ehrlichia chaffeensis and potential application for serodiagnosis

Journal of Clinical Microbiology

Volumn 38, Issue 10, 2000, Pages 3705-3709

  Alleman, A R ^a   Barbet, A F ^a   Bowie, M V ^a   Sorenson, H L ^a   Wong, S J ^a   Belanger, M ^a  

^a University of Florida   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MAJOR ANTIGENIC PROTEIN 2; UNCLASSIFIED DRUG;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; EHRLICHIA CHAFFEENSIS; EHRLICHIOSIS; GENE AMPLIFICATION; GENE EXPRESSION; GENE SEQUENCE; HUMAN; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SERODIAGNOSIS;

BACTERIA (MICROORGANISMS); EHRLICHIA; EHRLICHIA CHAFFEENSIS; RICKETTSIALES;

EID: 0033778939     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.10.3705-3709.2000     Document Type: Article

References (22)

1. Evaluation of Anaplasma marginale major surface protein 3 (MSP3) as a diagnostic test antigen
2. Potential value of major antigenic protein 2 for serological diagnosis of heartwater and related ehrlichial infections
3. Serologic diagnosis of human monocytic ehrlichiosis by immunoblot analysis
4. Identification of the antigenic constituents of Ehrlichia chaffeensis
5. Analysis and ultrastructural localization of Ehrlichia chaffeensis proteins with monoclonal antibodies
6. Human ehrlichiosis in the United States, 1985 to 1990
7. Serodiagnosis of human granulocytic ehrlichiosis by a recombinant HGE-44-based enzyme-linked immunosorbent assay
8. Serologic evidence for antigenic relationships between Ehrlichia canis and Cowdria ruminantium
9. Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle
10. Hemocytic rickettsia-like organisms in ticks: Serologic reactivity with antisera to Ehrlichiae and detection of DNA of agent of human granulocytic ehrlichiosis by PCR
11. Molecular cloning of a gene encoding the immunogenic 21 kDa protein of Cowdria ruminantium
12. Conformational dependence of Anaplasma marginale major surface protein 5 surface-exposed B-cell epitopes
13. Antigenic analysis of four species of the genus Ehrlichia by use of protein immunoblots
14. Cloning and characterization of multigenes encoding the immunodominant 30-kilodalton major outer membrane proteins of Ehrlichia canis and application of the recombinant protein for serodiagnosis
15. Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein
16. Western and dot blotting analyses of Ehrlichia chaffeensis indirect fluorescent-antibody assay-positive and -negative human sera by using native and recombinant E. chaffeensis and E. canis antigens
17. The Anaplasma marginale msp5 gene encodes a 19-kilodalton protein conserved in all recognized Anaplasma species
18. Diagnosing human ehrlichioses: Current status and recommendations
19. Serological responses to Ehrlichia equi, Ehrlichia chaffeensis, and Borrelia burgdorferi in patients from New York State
20. The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool
21. Comparison of Ehrlichia chaffeensis recombinant proteins for serologic diagnosis of human monocytic ehrlichiosis
22. MiniPrep in ten minutes