Tau protein is hyperphosphorylated in a site-specific manner in apoptotic neuronal PC12 cells

Journal of Neurochemistry

Volumn 75, Issue 6, 2000, Pages 2346-2357

  Zhang, Jianwen ^a   Johnson, Gail V W ^a,b  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

Author keywords

Apoptosis; Cdc2; Cdk5; Hyperphosphorylation; Tau

Indexed keywords

CYCLIN DEPENDENT KINASE; PROTEIN KINASE; TAU PROTEIN;

ALZHEIMER DISEASE; ANIMAL CELL; APOPTOSIS; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; MICROTUBULE; NERVE CELL NECROSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN PHOSPHORYLATION;

ANIMALS; APOPTOSIS; BINDING SITES; CDC2 PROTEIN KINASE; CYCLIN-DEPENDENT KINASE 5; CYCLIN-DEPENDENT KINASES; NEURONS; PC12 CELLS; PHOSPHORYLATION; PROTEIN SUBUNITS; PROTEIN-SERINE-THREONINE KINASES; RATS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TAU PROTEINS;

ANIMALIA;

EID: 0033777275     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2000.0752346.x     Document Type: Article

References (77)

1. Association of cyclindependent kinase 5 and its activator p35 with apoptotic cell death
2. Role of abnormally phosphorylated tau in the breakdown of microtubules in Alzheimer disease
3. Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: Correlation with suppression of endonuclease activity
4. Phosphorylation of Ser262 strongly reduces binding of tau to microtubules: Distinction between PHF-like immunoreactivity and microtubule binding
5. The distribution of tau in the mammalian central nervous system
6. Abnormal tau phosphorylation at Ser396 in Alzheimer's disease recapitulates development and contributes to reduced microtubule binding
7. Interaction of tau with the neural plasma membrane mediated by tau's amino-terminal projection domain
8. Ectopic cell cycle proteins predict the sites of neuronal cell death in Alzheimer's disease brain
9. Primary structure of high molecular weight tau present in the peripheral nervous system
10. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B
11. Energy metabolism and protein phosphorylation during apoptosis: A phosphorylation study of tau and high-molecular-weight tau in differentiated PC12 cells
12. The microtubule binding of tau and high molecular weight tau in apoptotic PC12 cells is impaired because of altered phosphorylation
13. Select alterations in protein kinases and phosphatases during apoptosis of differentiated PC12 cells
14. MAPs, MARKs and microtubule dynamics
15. Regulation of neuronal survival by the serine-threonine protein kinase Akt
16. Induction of cyclin B and H1 kinase activity in apoptotic PC12 cells
17. Involvement of caspases in proteolytic cleavage of Alzheimer's amyloid-beta precursor protein and amyloidogenic A beta peptide formation
18. Multiple isoforms of human microtubule-associated protein tau: Sequences and localization in neurofibrillary tangles of Alzheimer's disease
19. Cloning of a big tau microtubule-associated protein characteristic of the peripheral nervous system
20. Nerve growth factor prevents the death and stimulates the neuronal differentiation of clonal PC12 pheochromocytoma cells in serum-free medium
21. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor
22. Down-regulation of actin genes precedes microfilament network disruption and actin cleavage during p53-mediated apoptosis
23. The Alzheimer-like phosphorylation of tau protein reduces microtubule binding and involves Ser-Pro and Thr-Pro motifs
24. New phosphorylation sites identified in hyperphosphorylated tau (paired helical filament-tau) from Alzheimer's disease brain using nanoelectrospray mass spectrometry
25. Cell death and the cell cycle: A relationship between transformation and neurodegeneration?
26. Increased expression of cyclin-dependent kinase 5 in induced apoptotic neuron death in rat substantia nigra
27. Modulation of the glycogen synthase kinase-3 family by tyrosine phosphorylation
28. SAPK2/p38-dependent F-actin reorganization regulates early membrane blebbing during stress-induced apoptosis
29. The endogenous and cell cycle-dependent phosphorylation of tau protein in living cells: Implications for Alzheimer's disease
30. Possible role of tau protein kinases in pathogenesis of Alzheimer's disease
31. Tau protein kinase I converts normal tau protein into A68-like component of paired helical filaments
32. cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease
33. Tau protein in normal and Alzheimer's disease brain: An update
34. Tau protein in normal and Alzheimer's disease brain
35. Proteolysis of tau by calpain
36. Microtubule dynamics in living cells
37. Developmentally regulated expression of specific tau sequences
38. Casein kinase 1 is tightly associated with paired-helical filaments isolated from Alzheimer's disease brain
39. Calpain-dependent proteolytic cleavage of the p35 cyclin-dependent kinase 5 activator to p25
40. Implication of brain cdc2 and MAP2 kinases in the phosphorylation of tau protein in Alzheimer's disease
41. The primary structure and heterogeneity of tau protein from mouse brain
42. Neurotoxicity induces cleavage of p35 to p25 by calpain
43. Disruption of the cytoskeleton in Alzheimer's disease
44. Actin polymerization and depolymerization during apoptosis in HL-60 cells
45. p53, the cellular gatekeeper for growth and division
46. Phosphorylation affects the ability of tau protein to promote microtubule assembly
47. Tau in Alzheimer's disease
48. Characterization of tau phosphorylation in glycogen synthase kinase-3beta and cyclin dependent kinase-5 activator (p23) transfected cells
49. Activation of a PP2A-like phosphatase and dephosphorylation of tau protein characterize onset of the execution phase of apoptosis
50. Extranuclear apoptosis. The role of the cytoplasm in the execution phase
51. Proline-directed and non-proline-directed phosphorylation of PHF-tau
52. Aberrant tau phosphorylation and neurite retraction during NGF deprivation in PC12 cells
53. High resolution two-dimensional electrophoresis of basic as well as acidic proteins
54. Monoclonal antibody PHF-1 recognizes tau protein phosphorylated at serine residues 396 and 404
55. Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration
56. Accumulation of cyclin-dependent kinase 5 (cdk5) in neurons with early stages of Alzheimer's disease neurofibrillary degeneration
57. A system for characterizing cellular and molecular events in programmed neuronal cell death
58. Detection of phosphorylated Ser262 in fetal tau, adult tau, and paired helical filament tau
59. Interaction of neuronal Cdc2-like protein kinase with microtubule-associated protein tau
60. Death by design: Mechanism and control of apoptosis
61. Activation of caspase-3 in single neurons and autophagic granules of granulovacuolar degeneration in Alzheimer's disease. Evidence for apoptotic cell death
62. Mutations causing charge alterations in regulatory subunits of the cAMP-dependent protein kinase of cultured S49 lymphoma cells
63. Recognition of the minimal epitope of monoclonal antibody Tau-1 depends upon the presence of a phosphate group but not its location
64. Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators
65. Domains of tau protein, differential phosphorylation, and dynamic instability of microtubules
66. p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5
67. Plasma membrane alterations and cytoskeletal changes in apoptosis
68. Cell death in development
69. Mitotic mechanisms in Alzheimer's disease?
70. Aberrant expression of mitotic cdc2/cyclin B1 kinase in degenerating neurons of Alzheimer's disease brain
71. Proline-directed phosphorylation of human Tau protein
72. A protein factor essential for microtubule assembly
73. T-cell activation leads to rapid stimulation of translation initiation factor eIF2B and inactivation of glycogen synthase kinase-3
74. Tau in paired helical filaments is functionally distinct from fetal tau: Assembly incompetence of paired helical filament-tau
75. Substrate specificity of CDC2 kinase from human HeLa cells as determined with synthetic peptides and molecular modeling
76. Cyclin-dependent kinase 5 is associated with apoptotic cell death during development and tissue remodeling
77. Inhibition of Akt kinase by cell-permeable ceramide and its implications for ceramide-induced apoptosis