High-level expression of biologically active human prolactin from recombinant baculovirus in insect cells

Protein Expression and Purification

Volumn 20, Issue 2, 2000, Pages 265-273

  Das, Tapas ^a   Johns, Paul W ^a   Goffin, Vincent ^b   Kelly, Paul ^b   Kelder, Bruce ^c   Kopchick, John ^c   Buxton, Kristi ^a   Mukerji, Pradip ^a  

^a ABBOTT LABORATORIES   (United States)

^b INSERM   (France)

^c OHIO UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS; CARBOHYDRATE ANALYSIS; COMPLEMENTARY DNA; CULTURE MEDIUM; EXPRESSION VECTOR; INSECT CELL; POLYHEDRIN; PROLACTIN; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; VIRUS INFECTION;

EID: 0033769041     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1290     Document Type: Article

References (27)

1. Prolactin: Normal synthesis, regulation, and actions in 'Endocrinology'
2. Effects of growth hormone and prolactin immune development and function
3. Human prolactin: cDNA structural analysis and evolutionary comparisons
4. Structural variants of prolactin: Occurrence and physiological significance
5. Bioactive and immunoreactive prolactin variants in human milk
6. Do milkborne cytokines and hormones influence neonatal immune cell function?
7. Expression of prolactin (PRL) receptor gene and PRL-binding sites in rabbit intestinal epithelial cells
8. Use of neuroendocrine hormone to promote reconstitution after bone marrow transplantation
9. Prolactin is an antagonist of TGF-beta activity and promotes proliferation of murine B cell hybridomas
10. A review of the hormone prolactin during lactation
11. Opposing actions of intact and N-terminal fragments of the human prolactin/growth hormone family members on angiogenesis: An efficient mechanism for the regulation of angiogenesis
12. Bacterial production of recombinant human prolactin
13. 6-Ile-Glu-Gly-Arg-human prolactin (tag-hPRL) secreted in the periplasmic space of Escherichia coli
14. Studies on the microheterogeneity and in vitro activity of glycosylated and nonglycosylated recombinant human prolactin separated using a novel purification process
15. Principles and Practice of Protein Engineering
16. Expression of L protein of vesicular stomatis virus indiana serotype from recombinant baculovirus in insect cells: Requirement of a host factor(s) for its biological activity in vitro
17. Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas
18. Biological properties of human prolactin analogs depend not only on global hormone affinity, but also on the relative affinities of both receptors' binding sites
19. Antagonistic properties of human prolactin analogs that show paradoxical agonistic activity in the Nb2 bioassay
20. Mammary gland factor (MGF) is a novel factor of the cytokines regulated transcription factor gene family and confers the prolactin response
21. Prolactin and its receptor: Actions, signal transduction pathways and phenotypes observed in PRL receptors knockout mice
22. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
23. Monosaccharide analysis of glycoconjugates by anion exchange chromatography with pulsed amperometric detection
24. A new sensitive and specific bioassay for lactogenic hormones: Measurment of prolactin and growth hormone in human serum
25. Heparin-binding property of human prolactin: A novel aspect of prolactin biology
26. Purification and interfacial behavior of recombinant human gastric lipase produced from insect cells in a bioreactor