Spontaneous blastomere fusion after freezing and thawing of early human embryos leads to polyploidy and chromosomal mosaicism

Human Reproduction

Volumn 15, Issue 11, 2000, Pages 2404-2410

  Balakier, Hanna ^a,c   Cabaca, Oliver ^a   Bouman, Derek ^b   Shewchuk, Alan B ^a   Laskin, Carl ^a,b   Squire, Jeremy A ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c Success Through Assisted Reproductive Technologies (START)   (Canada)

Author keywords

Cell fusion; Chromosomal aberrations; Cryopreservation; Mosaicism; Polypolidy

Indexed keywords

BLASTOMERE; CHROMOSOME ABERRATION; CHROMOSOME ANALYSIS; CHROMOSOME MOSAICISM; CONFERENCE PAPER; CRYOPRESERVATION; EMBRYO; EMBRYO DEVELOPMENT; EMBRYO TRANSFER; FERTILIZATION IN VITRO; FLUORESCENCE IN SITU HYBRIDIZATION; GENE FUSION; GENE SEQUENCE; GENE TARGETING; HUMAN; HUMAN CELL; INFERTILITY; INTRACYTOPLASMIC SPERM INJECTION; MAJOR CLINICAL STUDY; MULTINUCLEAR CELL; POLYPLOIDY; SURVIVAL RATE; THAWING; TREATMENT OUTCOME;

EID: 0033768915     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/15.11.2404     Document Type: Conference Paper

References (29)

1. The effect of cryopreservation on development of S- and G2-phase mouse embryos
2. Chromosome analysis of two-cell mouse embryos frozen by slow and ultrarapid methods using two different cryoprotectants
3. Intercellular communication in the early human embryo
4. Comparaison des grossesses issues de transferts d'embryons congeles aux grossesses issues de transferts d'embryons frais en fecondation in vitro
5. The protective effects of polymers in the cryopreservation of human and mouse zonae pellucidae and embryos
6. Tetraploidy after frozen embryo transfer: Cryopreservation may interfere with first mitotic division
7. Tetraploidy in mice, embryonic cell number, and the grain of the developmental map
8. Membrane fusion through point defects in bilayers
9. The optimal equilibration time for mouse embryos frozen by vitrification with trehalose
10. A chimaeric animal model for confined placental mosaicism
11. Chromosomal analysis of unfertilized human oocytes prepared by a gradual fixation-air drying method
12. Fluorescent in situ hybridization on human embryos showing cleavage arrest after freezing and thawing
13. Ultrarapid detection of sex chromosomes with the use of fluorescence in situ hybridization with direct label DNA probes in single human blastomeres, spermatozoa, amniocytes, and lymphocytes
14. Lineage tracing demonstrates that blastomeres of early cleavage-stage human pre-embryos contribute to both trophectoderm and inner cell mass
15. Fine structure of early human embryos frozen with 1,2 propanediol
16. Perinatal outcome and follow-up of 82 children aged 1-9 years old conceived from cryopreserved embryos
17. Edward's syndrome after the replacement of cryopreserved-thawed embryos
18. Embryo quality and pregnancy potential of fresh compared with frozen embryos: Is freezing detrimental to high quality embryos?
19. An association between chromosomal abnormalities in rapidly frozen 2-cell mouse embryos and the ice-forming properties of the cryoprotective solution
20. Tetraploid conceptus with three paternal contributions
21. Minor congenital anomalies, major congenital malformations and development in children conceived from cryopreserved embryos
22. High pregnancy rate after early human embryo freezing
23. In vitro fertilization and embryo preservation
24. Birth characteristics and perinatal outcome of babies conceived from cryopreserved embryos
25. Obstetric and perinatal outcome of children conceived from cryopreserved embryos
26. Embryo freezing: Is it safe?
27. Electrofusion of cells: State of the art and future directions
28. Electric field-induced cell-to-cell fusion