Specific determination of endothelial cell viability in the whole cell fraction from cryopreserved canine femoral veins using flow cytometry

Artificial Organs

Volumn 24, Issue 10, 2000, Pages 829-833

  Park, Jong Chul ^a   Sung, Hak Joon ^a   Lee, Dong Hee ^a   Park, Young Hwan ^a   Cho, Bum Koo ^a   Suh, Hwal ^a  

^a Yonsei University College of Medicine   (South Korea)

Author keywords

Cardiovascular vein; Cryopreservation; Endothelial cells; Griffonia simplicifolia agglutins propidium iodide flow cytometry analytical method; Simultaneous viability test

Indexed keywords

BIOLOGICAL MATERIALS PRESERVATION; CYTOLOGY; FLOW CYTOMETRY; THAWING;

ANALYTICAL METHOD; CARDIOVASCULAR VEIN; CRYO-PRESERVATION; ENDOTHELIAL-CELLS; FEMORAL VEIN; GRIFFONIA SIMPLICIFOLIUM AGGLUTIN/PROPIDIUM IODIDE FLOW CYTOMETRY ANALYTICAL METHOD; HUMAN SAPHENOUS VEIN; PROPIDIUM IODIDE; SIMULTANEOUS VIABILITY TEST; WHOLE CELL;

ENDOTHELIAL CELLS;

FLUORESCEIN ISOTHIOCYANATE; PROLINE; PROPIDIUM IODIDE; TRYPAN BLUE;

ADULT; ANIMAL TISSUE; ARTICLE; CELL VIABILITY; CRYOPRESERVATION; DOG; ENDOTHELIUM CELL; FEMORAL VEIN; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; CANIS FAMILIARIS; GRIFFONIA SIMPLICIFOLIA;

EID: 0033764721     PISSN: 0160564X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1525-1594.2000.06514-2.x     Document Type: Article

References (8)

1. Production of Interleukin 1 by human endothelial cells
2. Endothelial cell viability in the rat aortic wall
3. Viability and enzyme activity of cryopreserved porcine heart valve
4. Isolation and characterization of macrovascular and microvascular endothelial cells from human hearts
5. An improved method to determine cell viability by simultaneous staining with fluorescein diacetatepropidium iodide
6. Establishing a protocol to quantify leaflet fibroblast responses to physiologic flow through a viable heart valve
7. Purification of murine endothelial cell cultures by flow cytometry using fluorescein-labeled griffonia simplicifollia agglutinin
8. Freezing of living cells: Mechanisms and implication