Two-dimensional gel electrophoresis analyses of pH-dependent protein expression in facultatively alkaliphilic Bacillus pseudofirmus OF4 lead to characterization of an S-layer protein with a role in alkaliphily

Journal of Bacteriology

Volumn 182, Issue 21, 2000, Pages 5969-5981

  Gilmour, R ^a   Messner, P ^a   Guffanti, A A ^a   Kent, R ^a   Scheberl, A ^a   Kendrick, N ^a   Krulwich, T A ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINO 2 METHYLPROPIONIC ACID; ADENOSINE TRIPHOSPHATE; ARGININE; BACTERIAL PROTEIN; LYSINE; MEMBRANE PROTEIN;

ALKALINITY; ARTICLE; BACILLUS; BACILLUS PSEUDOFIRMUS; BACTERIAL GROWTH; BACTERIUM MUTANT; ELECTRON MICROSCOPY; ENERGY COST; GLUCOSE UTILIZATION; GROWTH RATE; HOMEOSTASIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; STRAIN DIFFERENCE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ALKALIES; AMINO ACID SEQUENCE; ARGININE; BACILLUS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BASE SEQUENCE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HYDROGEN-ION CONCENTRATION; LYSINE; MOLECULAR SEQUENCE DATA; MUTATION; RNA, BACTERIAL;

BACILLUS PSEUDOFIRMUS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0033764479     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.21.5969-5981.2000     Document Type: Article

References (48)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis
3. Isolation and partial characterization of structural components of the walls of alkalophilic Bacillus strain C-125
4. A high cell wall negative charge is necessary for the growth of the alkaliphile Bacillus lentus C-125 at elevated pH
5. Contribution of the cell wall component teichuronopeptide to pH homeostasis and alkaliphily in the alkaliphilic Bacillus lentus C-125
6. Specific and general stress proteins in Bacillus subtilis - A two-dimensional protein electrophoresis study
7. Role of cellular design in bacterial metal accumulation and mineralization
8. Functions of S-layers
9. Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824
10. Mutation of Bacillus firmus OF4 to duramycin resistance results in substantial replacement of membrane lipid phosphatidylethanolamine by its plasmalogen form
11. Membrane lipid composition of obligately and facultatively alkalophilic strains of Bacillus spp
12. Initiation of mRNA decay in Bacillus subtilis
13. Surface proteins and a novel transcription factor regulate the expression of the S-layer gene in Thermus thermophilus HB8
14. Purification and characterization of flagella from the alkalophile Bacillus firmus RAB
15. Molar growth yields and bioenergetic parameters of extremely alkaliphilic Bacillus species in batch cultures, and growth in a chemostat at pH 10.5
16. Features of apparent nonchemiosmotic energization of oxidative phosphorylation by alkaliphilic Bacillus firmus OF4
17. Oxidative phosphorylation by ADP + P(i)-loaded membrane vesicles of alkaliphilic Bacillus firmus OF4
18. + antiporter system of alkalophilic Bacillus species strain C-125
19. Techniques for transformation of E. coli
20. Alkaliphiles: Some applications of their products for biotechnology
21. +-uptake-deficient Escherichia coli include an ftsH homologue
22. + antiporter of alkaliphilic Bacillus firmus OF4
23. Microbial diversity of soda lakes
24. Glycan structure of the S-layer glycoprotein of Bacillus sp. L420-L491
25. Alkaliphilic prokaryotes
26. pH tolerance in Bacillus: Alkaliphile vs non-alkaliphile
27. Energetics of alkaliphilic Bacillus species: Physiology and molecules
28. pH homeostasis and ATP synthesis: Studies of two processes that necessitate inward proton translocation in extremely alkaliphilic Bacillus species
29. The complete genome sequence of the gram-positive bacterium Bacillus subtilis
30. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
31. Domain structure of the Acetogenium kivui surface layer revealed by electron crystallography and sequence analysis
32. Description of Bacillus thermoaerophilus sp. nov., to include sugar beet isolates and Bacillus brevis ATCC 12990
33. The capsule and S-layer: Two independent and yet compatible macromolecular structures in Bacillus anthracis
34. The proteome of Salmonella enterica serovar Typhimurium: Current progress on its determination and some applications
35. High resolution two dimensional electrophoresis of proteins
36. Biosynthesis of branched-chain fatty acids in Bacillus subtilis: A decarboxylase is essential for branched-chain fatty acid synthetase
37. S-layer proteins
38. Dynamics in oxygen-induced changes in S-layer protein synthesis from Bacillus stearothermophilus PV72 and the S-layer-deficient variant T5 in continuous culture and studies of the cell wall composition
39. Analysis of crystalline bacterial cell surface layers by freeze-etching, metal shadowing, negative staining, and ultrathin sectioning
40. Crystalline bacterial cell surface layers
41. Growth and bioenergetics of alkaliphilic Bacillus firmus OF4 in continuous culture at high pH
42. + re-entry pathway in alkaliphilic bacteria
43. Sequence completion, identification and definition of the fengycin operon in Bacillus subtilis 168
44. Gene protein database of E. coli K-12, edition
45. Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene
46. The primary structure of branched-chain alpha-oxo acid dehydrogenase from Bacillus subtilis and its similarity to other alpha-oxo acid dehydrogenases
47. Peptide antibiotics