Variability in E-selectin expression, mRNA levels and sE-selectin release between endothelial cell lines and primary endothelial cells

Cell Biology International

Volumn 24, Issue 2, 2000, Pages 91-99

  Galley, H F ^a   Blaylock, M G ^a   Dubbels, A M ^a   Webster, N R ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

Adhesion molecule; E selectin; Endothelial cells; Leucocyte

Indexed keywords

ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE 1; INTERLEUKIN 1BETA; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL LINE; CONTROLLED STUDY; ENDOTHELIUM CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; NEUTROPHIL; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECRETION; UMBILICAL VEIN;

ANIMALIA;

EID: 0033758674     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1006/cbir.1999.0455     Document Type: Article

References (38)

1. E-selectin supports neutrophil rolling in vitro under conditions of flow
2. A permanent human cell line (EA.hy926) reserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells
3. Hyaluronic acid synthesis is absent in normal human endothelial cells irrespective of hyaluronic acid synthetase inhibitor activity, but is significantly high in transformed cells
4. Cultivation of endothelial cells: Limitations and perspectives
5. Growth characteristics of cultured human macrovascular venous and arterial and microvascular endothelial cells
6. Endothelial leukocyte adhesion molcule-1: An inducible receptor for neutrophils related to complement regulatory proteins and lectins
7. Regulation of ICAM-1 by dexamethasone in a human vascular endothelial cell line EA.hy926
8. Tumour necrosis factor-alpha induced ICAM-1 expression in human vascular and lung epithelial cells: Modulation by tyrosine kinase inhibitors
9. The expression and release of adhesion molecules by human endothelial cell lines and their consequent binding of lymphocytes
10. Characterization of a spontaneously transformed human endothelial cell line
11. Differential expression of the common beta and specific alpha chain of the receptors for GM-CSF, IL-13 and IL-5 in endothelial cells
12. A permanent cell line expressing factor VIII related antigen established by hybridisation
13. Endothelium-specific Weibel-Palade bodies in a continuous human cell line, EA.hy926
14. Effect of ciprofloxacin on the accumulation of interleukin-6, interleukin-8 and nitrite from an endothelial cell model of sepsis
15. Nitric oxide production by human peripheral blood polymorphonuclear leucocytes
16. Methods for the detection of anti-endothelial antibodies by enzyme-linked immunosorbent assay
17. Probucol downregulates E-selectin expression on cultured human vascular endothelial cells
18. Comparative studies on vascular endothelium in vitro
19. Chylomicrons induce E-selectin and VCAM expression in endothelial cells
20. Immunologic phenotype of cultured endothelial cells: Quantitative analysis of cell surface molecules
21. Re-evaluation of trypsin-EDTA for endothelial detachment before flow cytometry analysis
22. Soluble E-selectin is found in supernatants of activated endothelial cells and is elevated in the serum of patients with septic shock
23. A monoclonal antibody to the human leukocyte adhesion molecule intercellular adhesion molecule-2. Cellular distribution and molecular characterisation of the antigen
24. Role of N-linked glycosylation in expression of E-selectin on human endothelial cells
25. Two distinct monokines, interleukin-1 and tumor necrosis factor, each independently induce biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells
26. Induction of cyclo-oxygenase-2 by interleukin-1 alpha. Evidence for post transcriptional regulation
27. A factor in human plasma permits persistent expression of E-selectin by human endothelial cells
28. Potential significance of circulating E-selectin
29. Rare spontaneously transformed human endothelial cell line provides useful research tool
30. IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor and IFN-gamma
31. IL-4 increases human endothelial cell adhesiveness for T cells but not for neutrophils
32. Leucocyte endothelial cell adhesion: A study comparing human umbilical vein endothelial Cells and the endothelial cell line EA-hy-926
33. Neutrophil-endothelial cell interactions: Mechanisms of neutrophil adherence to vascular endothelium
34. Isolation of a human von Willebrand factor cDNA from the hybrid endothelial cell line EA.hy926
35. Nitric oxide regulation of IL-8 expression in human endothelial cells
36. Expression of ECE and related membrane peptidases in the EA.hy926 cell line
37. Dexamethasone inhibits cytokine induced intercellular adhesion molcule-1 up-regulation on endothelial cell lines
38. Unique expression of von Willebrand factor by type IIA von Willebrand's disease endothelial cells