Multiple forms of the serine protease Alp of Aspergillus fumigatus

Mycoses

Volumn 43, Issue 9-10, 2000, Pages 339-347

  Kunert, J ^a   Kopecek, P ^a  

^a PALACKÝ UNIVERSITY   (Czech Republic)

Author keywords

Aspergillus fumigatus; Isoenzymes; Proteases

Indexed keywords

ALKALINE PROTEINASE; CALCIUM; FUNGAL ENZYME; GLUCOSE; ISOENZYME; PEPTIDE; PROTEIN; PYROXYLIN; SERINE PROTEINASE; SUBTILISIN;

ARTICLE; ASPERGILLUS FUMIGATUS; ENZYME ANALYSIS; ENZYME DENATURATION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STABILITY; FUNGUS CULTURE; FUNGUS GROWTH; GEL CHROMATOGRAPHY; ISOELECTRIC FOCUSING; ISOELECTRIC POINT; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; TEMPERATURE;

ANIMALS; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; CHICKENS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOBLOTTING; ISOELECTRIC FOCUSING; ISOENZYMES; LUNG DISEASES, FUNGAL; POULTRY DISEASES; SERINE ENDOPEPTIDASES;

EID: 0033756488     PISSN: 09337407     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1439-0507.2000.00586.x     Document Type: Article

References (42)

1. Purification and characterization of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue
2. Purification and properties of the elastase from Aspergillus fumigatus
3. Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline proteinase of Aspergillus famigatus
4. Evidence for possible involvement of an elastolytic serine protease in aspergillosis
5. Purification and characterization of a fibrinogenolytic serine proteinase from Aspergillus fumigatus culture filtrate
6. Isolation and characterization of an extracellular alkaline protease of Aspergillus fumigatus
7. An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity
8. Virulence of alkaline protease-deficient mutants of Aspergillus fumigatus
9. The alkaline protease of Aspergillus fumigatus is not a virulence determinant in two murine models of invasive pulmonary aspergillosis
10. Purification and characterization of an elastinolytic metalloprotease from Aspergillus fumigatus and immunoelectron microscopic evidence of secretion of this enzyme by the fungus invading the murine lung
11. Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus
12. Cloning and disruption of the gene encoding an extracellular metalloprotease of Aspergillus fumigatus
13. Molecular cloning and sequencing of the cDNA and gene for a novel elastinolytic metalloproteinase from Aspergillus fumigatus and its expression in Escherichia coli
14. Chromatographic purification and homogeneity of extracellular acid proteinase of Aspergillus fumigatus
15. Purification and characterization of an extracellular aspartic proteinase from Aspergillus fumigatus
16. Molecular cloning of the cDNA and gene for an elastinolytic aspartic proteinase from Aspergillus fumigatus and evidence of its secretion by the fungus during invasion of the host lung
17. Virulence of an aspergillopepsin-deficient mutant of Aspergillus fumigatus and evidence for another aspartic proteinase linked to the fungal cell wall
18. Disruption of the serine proteinase gene (sep) in Aspergillus flavus leads to a compensatory increase in the expression of a metalloprotease gene (mep 20)
19. Biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from Aspergillus fumigatus
20. Dipeptidyl-peptidase IV secreted by Aspergillus fumigatus, a fungus pathogenic to humans
21. Cloning and characterization of the cDNAs and genes (mep 20) encoding homologous metalloproteinases from Aspergillus flavus and A. fumigatus
22. Growth and production of extracellular proteases by the fungus Aspergillus fumigatus on various media. I. Media without proteins
23. The use of Ink Blue in the identification of dermatophytes
24. Enzymoblotting: A method for localizing proteinases and their zymogens using para-nitroanilide substrates after agarose gel electrophoresis and transfer to nitrocellulose
25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
26. Methods for increasing resolution of two-dimensional protein electrophoresis
27. Zymographic techniques for detection and characterization of microbial proteases
28. Preliminary characterization of extracellular enzymes of dermatophytes by chromogenic substrates
29. Application de la chromatographie d'affinite a l'isolement des fractions antigeniques d'Aspergillus fumigatus supportant une activite chymotrypsique
30. A study of alkaline proteases secreted by different Aspergillus species
31. Extracellular fibrinogenolytic enzyme of Aspergillus fumigatus: Substrate-dependent variations in the proteinase synthesis and characterization of the enzyme
32. Action of Aspergillus serine proteinase on fluorogenic and chromogenic substrates
33. Some properties of the alkaline proteinase from Aspergillus melleus
34. Isolation, characterization, and cloning of cDNA and the gene for an elastinolytic serine proteinase from Aspergillus flavus
35. A 33 kDa proteinase from Scedosporium apiospermum
36. Isolation and properties of the serine protease from Aspergillus oryzae
37. Immunologic significance of a collagen-derived culture filtrate containing proteolytic activity in Aspergillus-related diseases
38. Anomalous estimation of protease molecular weights using gelatin-containing SDS-PAGE
39. Isolation and characterization of the alkaline protease gene of Aspergillus oryzae
40. Recombinant expression and antigenic properties of a 32-kilodalton extracellular alkaline protease, representing a possible virulence factor from Aspergillus fumigatus
41. Identification of a 33kDa alkaline protease of Aspergillus fumigatus in vitro and in vivo
42. Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus