Monitoring genome evolution ex vivo: Reversible chromosomal integration of a 106 kb plasmid at two tRNA(Lys) gene loci in sequential Pseudomonas aeruginosa airway isolates

Microbiology

Volumn 146, Issue 10, 2000, Pages 2365-2373

  Kiewitz, C ^a   Larbig, K ^a   Klockgether, J ^a   Weinel, C ^a   Tummler, B ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Cystic fibrosis; Genome evolution; Pseudomonas aeruginosa; Recombination; TRNA gene

Indexed keywords

BACTERIAL DNA; CARRIER PROTEIN; LYSINE TRANSFER RNA; PYOCIN;

AIRWAY; ARTICLE; BACTERIAL MUTATION; BACTERIOPHAGE; BACTERIUM ISOLATION; CHROMOSOME INSERTION; CONTROLLED STUDY; CYSTIC FIBROSIS; DNA DETERMINATION; DNA STRAND BREAKAGE; GENE DELETION; GENE INSERTION; GENE LOCUS; GENE SEQUENCE; GENETIC VARIABILITY; HUMAN; MOLECULAR CLONING; MOLECULAR EVOLUTION; MOSAICISM; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN SYNTHESIS INHIBITION; PSEUDOMONAS AERUGINOSA; RESTRICTION MAPPING; SPECIES DIFFERENCE; STRAIN DIFFERENCE; STRUCTURAL GENE;

PSEUDOMONAS AERUGINOSA;

EID: 0033753983     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-10-2365     Document Type: Article

References (34)

1. Plasmid integration in a wide range of bacteria mediated by the integrase of Lactobacillus delbrueckii bacteriophage mv4
2. Unusual structure of the attB site of the site-specific recombination system of Lactobacillus delbrueckii bacteriophage mv4
3. Heuristic approach to deriving models for gene finding
4. The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survival
5. Characterization of the genes and attachment sites for site-specific integration of plasmid pSE101 in Saccharopolyspora erythraea and Streptomyces lividans
6. Bacterial genomes: Physical structure and analysis
7. The site-specific integration system of the temperate Streptococcus thermophilus bacteriophage Sfi21
8. Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster
9. Bacteriophages
10. The diverse and dynamic structure of bacterial genomes
11. A simple and rapid method for the preparation of gram-negative bacterial genomic
12. New approaches in genome analysis by pulsed-field gel electrophoresis: Application to the analysis of Pseudomonas species
13. Pathogenicity islands of virulent bacteria: Structure, function and impact on microbial evolution
14. Molecular analysis of a cytotoxin-converting phage, CTX, of Pseudomonas aeruginosa: Structure of the attP-cos-ctx region and integration into the serine tRNA gene
15. Smith/Birnstiel mapping of genome rearrangements in Pseudomonas aeruginosa
16. Cloning of prokaryotic genomes in yeast artificial chromosomes: Application to the population genetics of Pseudomonas aeruginosa
17. Transfer RNAs and pathogenicity islands
18. Sequence diversity of Pseudomonas aeruginosa: Impact on population structure and genome evolution
19. Nucleotide sequence of the Pseudomonas aeruginosa insertion sequence IS222: Another member of the IS3 family
20. Genomic mapping with I-CeuI, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria
21. tRNAscan-SE: A program for improved detection of transfer RNA genes in genomic sequence
22. The complete nucleotide sequence of CTX, a cytotoxin-converting phage of Pseudomonas aeruginosa: Implications for phage evolution and horizontal gene transfer via bacteriophages
23. Genomic evolution during a 10,000-generation experiment with bacteria
24. Structure of the chromosomal insertion site for pSAM2: Functional analysis in Escherichia coli
25. Comparative mapping of the Pseudomonas aeruginosa PAO genome with rare-cutter linking clones or two-dimensional pulsed-field gel electrophoresis protocols
26. Epidemiology of chronic Pseudomonas aeruginosa infections in cystic fibrosis
27. Gradient of genomic diversity in the Pseudomonas aeruginosa chromosome
28. Large genome rearrangements discovered by the detailed analysis of 21 Pseudomonas aeruginosa clone C isolates found in environment and disease habitats
29. Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats
30. Comparison of type IV-pilin genes of Pseudomonas aeruginosa of various habitats has uncovered a novel unusual sequence
31. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea
32. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice
33. Cystic fibrosis: An inherited susceptibility to bacterial respiratory infections
34. Site-specific integration of bacteriophage VWB genome into Streptomyces venezuelae and construction of a VWB-based integrative vector