Limitations in the use of low pH extraction to distinguish internalized from cell surface-bound radiolabeled antibody

Nuclear Medicine and Biology

Volumn 27, Issue 6, 2000, Pages 571-575

  Ong, Gaik Lin ^a   Mattes, M Jules ^a  

^a CENTER FOR MOLECULAR MEDICINE AND IMMUNOLOGY   (United States)

Author keywords

Antibody internalization; Intracellular uptake; Low pH extraction

Indexed keywords

ANIMAL CELL; ANTIBODY DETECTION; ARTICLE; CELL PH; CELL SURFACE; INTERNALIZATION; ISOTOPE LABELING; LYSOSOME; NONHUMAN;

ACIDS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; CARCINOMA; CELL ADHESION; CELL MEMBRANE; CELL SURVIVAL; CHROMIUM RADIOISOTOPES; FEMALE; HUMANS; HYDROGEN-ION CONCENTRATION; INTRACELLULAR FLUID; LIGANDS; LYMPHOMA, B-CELL; LYSOSOMES; TRYPAN BLUE; TUMOR CELLS, CULTURED; UTERINE CERVICAL NEOPLASMS;

EID: 0033753780     PISSN: 09698051     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-8051(00)00145-1     Document Type: Article

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13. Cytotoxicity of CD3-ricin A chain immunotoxins in relation to cellular uptake and degradation kinetics
14. Rate of internalization of an immunotoxin correlates with cytotoxic activity against human tumor cells