Chemical degradation of tobacco mosaic virus followed by infectivity assay, reverse transcription-polymerase chain reaction and gel electrophoresis

Acta Virologica

Volumn 44, Issue 3-4, 2000, Pages 145-149

  Choi, C W ^a   Park, S H ^a   Choi, J K ^a   Ryu, K H ^a   Park, W M ^a  

^a Pal Chai University   (South Korea)

Author keywords

Chemical degradation; Gel electrophoresis; Infectivity; RT PCR; Tobacco mosaic virus; UV irradiation

Indexed keywords

DISINFECTANT AGENT; FORMALDEHYDE; PHENOL; SODIUM HYDROXIDE; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; GEL ELECTROPHORESIS; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION; TOBACCO MOSAIC VIRUS; VIRION; VIRUS INFECTIVITY; VIRUS PARTICLE;

ELECTROPHORESIS, AGAR GEL; FORMALDEHYDE; HYDROCHLORIC ACID; PHENOL; PLANT DISEASES; PLANTS, TOXIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SODIUM HYDROXIDE; TIME FACTORS; TOBACCO; TOBACCO MOSAIC VIRUS; VIRION;

NICOTIANA RUSTICA; NICOTIANA TABACUM; TOBACCO MOSAIC VIRUS;

EID: 0033753161     PISSN: 0001723X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Detection of tobamoviruses from purified or crude extracts after agarose gel electrophoresis
2. Epidemiology and control of tomato mosaic virus
3. Transmission, movement, and inactivation of cymbidium mosaic and odontoglossum ringspot viruses
4. Mechanism for release of RNA from turnip yellow mosaic virus at high pH
5. Nucleotide sequence of cDNA of the tobacco mosaic virus RNA in Korea
6. Persistence of tomato mosaic virus in tomato debris and soil under filed conditions
7. Cell culture and PCR determination of poliovirus inactivation by disinfectants
8. Effect of biocides on MS2 and K coliphages
9. Partial characterization of a soybean strain of tobacco mosaic virus
10. Efficacy of nucleic acid probes for detection of poliovirus in water disinfected by chlorine, chlorine dioxide, ozone, and UV radiation
11. The characterization of intermediates formed during the disassembly of tobacco mosaic virus at alkaline pH
12. The polarity of stripping of coat protein subunits from the RNA in tobacco mosaic virus under alkaline conditions
13. In situ inactivation of animal viruses and a coliphage in nonaerated liquid and semiliquid animal wastes
14. Efficacy of chemical disinfectants against turbot aquareovirus
15. Rapid detection and characterization of plant viruses by agarose gel electrophoresis: Size, surface charge and heterogeneity of panicum mosaic and related viruses
16. Modification of the coat protein charge and its effect on the stability of the U1 strain of tobacco mosaic virus at alkaline pH
17. Inactivation of coliphage MS-2 and poliovirus by copper, silver, and chlorine