Application of molecular methods to microbial community analysis of activated sludge

Water Science and Technology

Volumn 42, Issue 3-4, 2000, Pages 17-22

  Onuki, M ^a   Satoh, H ^a   Mino, T ^a   Matsuo, T ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Activated sludge; Community analysis; FISH; Molecular methods; PCR DGGE; Sequencing

Indexed keywords

BACTERIA; DNA; ELECTROPHORESIS; ENZYMES; GENETIC ENGINEERING; MICROSCOPIC EXAMINATION; NITRIFICATION; WASTEWATER TREATMENT; WATER BACTERIOLOGY;

DENATURING GRADIENT GEL ELECTROPHORESIS; FLUORESCENCE MICROSCOPY; FLUORESCENT IN SITU HYBRIDIZATION; MICROBIAL COMMUNITY ANALYSIS; MOLECULAR METHOD; POLYMERASE CHAIN REACTION; WASTEWATER TREATMENT PLANTS;

ACTIVATED SLUDGE PROCESS;

ACTIVATED SLUDGE; BACTERIAL FLORA; BACTERIUM IDENTIFICATION; CONFERENCE PAPER; DENATURING GRADIENT GEL ELECTROPHORESIS; IN SITU HYBRIDIZATION; METHODOLOGY; NITRIFICATION; NITROSOMONAS; POLYMERASE CHAIN REACTION; REACTOR; WASTE WATER MANAGEMENT;

NITROSOMONAS;

EID: 0033753050     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.2166/wst.2000.0353     Document Type: Conference Paper

References (12)

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4. Identifying members of the domain Archaea with rRNA-targeted oligonucleotide probes
5. Dual staining of natural bacterioplankton with 4',6-diamidino-2-phenlindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences
6. Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: Problems and solutions
7. In situ characterization of the microbial consortia active in two wastewater treatment plants
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9. In situ probing of Gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides
10. Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
11. Probing activated sludge with oligonucleotides specific for proteobacteria: Inadequacy of culture-dependent methods for describing microbial community structure
12. Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride