Simultaneous overexpression of enzymes of the lower part of glycolysis can enhance the fermentative capacity of Saccharomyces cerevisiae

Yeast

Volumn 16, Issue 14, 2000, Pages 1325-1334

  Smits, Hans Peter ^a   Hauf, Jörg ^c   Müller, Susanne ^c   Hobley, Timothy J ^b   Zimmermann, Friedrich K ^c   Hahn Hägerdal, Bärbel ^b   Nielsen, Jens ^a   Olsson, Lisbeth ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b LUND UNIVERSITY   (Sweden)

^c DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Fermentative capacity; Glyceraldehyde 3 phosphate dehydrogenase; Glycolytic flux; Lower glycolysis; Phosphoglycerate kinase; Pyruvate decarboxylase; Pyruvate kinase; Saccharomyces cerevisiae

Indexed keywords

ADENOSINE TRIPHOSPHATE; ALCOHOL; ALCOHOL DEHYDROGENASE; CARBON DIOXIDE; ENOLASE; GALACTOSE; GLUCOSE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GLYCOLYTIC ENZYME; PHOSPHOGLYCERATE KINASE; PHOSPHOGLYCERATE MUTASE; PYRUVATE DECARBOXYLASE; PYRUVATE KINASE;

ARTICLE; CONCENTRATION RESPONSE; ENZYME ACTIVITY; FERMENTATION; GLYCOLYSIS; HOST; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

AEROBIOSIS; ANAEROBIOSIS; BIOREACTORS; CARBON DIOXIDE; CULTURE MEDIA; ETHANOL; FERMENTATION; GALACTOSE; GENE EXPRESSION; GLUCOSE; GLYCOLYSIS; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0033753004     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0061(200010)16:14<1325::AID-YEA627>3.0.CO;2-E     Document Type: Article

References (36)

1. Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: Identification of alcohol dehydrogenase II as a stress protein
2. Cloning of a second gene encoding 6-phosphofructo-2-kinase in yeast and characterization of mutant strains without fructose-2,6-bisphosphate
3. 31P NMR magnetization-transfer measurements of flux between inorganic phosphate and adenosine 5'-triphosphate in yeast cells genetically modified to overproduce phosphoglycerate kinase
4. Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae
5. Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae
6. Strategics for manipulating metabolic fluxes in biotechnology
7. Metabolic control analysis of glycolysis and branching to ethanol production in chemostat cultures of Saccharomyces cerevisiae under carbon, nitrogen, or phosphate limitations
8. Effects of overexpression of phosphofructokinase on glycolysis in the yeast Saccharomyces cerevisiae
9. Optimization of product formation in a microbial fermentation process
10. Carbohydrate metabolism
11. Fermentation pathway kinetics and metabolic flux control in suspended and immobilized Saccharomyces cerevisiae
12. Simultaneous genomic overexpression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae
13. Isolation and characterization of the two structural genes coding for phosphofructokinase in yeast
14. Cooperation of glycolytic enzymes
15. +-ATPase and inverse respiratory control in Escherichia coli
16. Metabolic control theory: Its role in microbiology and biotechnology
17. Catabolite inactivation of the high-affinity hexose transporters Hxt6 and Hxt7 of Saccharomyces cerevisiae occurs in the vacuole after internalization by endocytosis
18. Mechanisms of appearance of the Pasteur effect in Saccharomyces cerevisiae: Inactivation of sugar transport systems
19. A novel signal transduction pathway in Saccharomyces cerevisiae defined by Snf3-regulated expression of HXT6
20. A kinetic study of glycolytic enzyme synthesis in yeast
21. Mutant studies of phosphofructo-2-kinase do not reveal an essential role of fructose-2,6-bisphosphate in the regulation of carbon fluxes in yeast cells
22. Futile cycles in Saccharomyces cerevisiae strains expressing the gluconeogenic enzymes during growth on glucose
23. The regulatory characteristics of yeast fructose-1,6-bisphosphatase confer only a small selective advantage
24. A strategy for increasing an in vivo flux by genetic manipulations. The tryptophan system of yeast
25. Kinetic characterization of individual hexose transporters of Saccharomyces cerevisiae and their relation to the triggering mechanisms of glucose repression
26. In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae. II. Mathematical model
27. Control of glucose metabolism by enzyme II(glc) of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coli
28. Overproduction of glycolytic enzymes in yeast
29. Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis
30. Clean-up and analysis of sugar phosphates in biological extracts by using solid phase extraction and anion-exchange chromatography with pulsed amperometric detection
31. Protein burden in Zymomonas mobilis: Negative flux and growth control due to overproduction of glycolytic enzymes
32. Effect of benzoic acid on metabolic fluxes in yeast: A continuous culture study on the regulation of respiration and alcoholic fermentation
33. Preparation and assay of deoxyribonucleic acids from animal tissue