Deletion of a conserved dinucleotide inhibits the second step of group II intron splicing

RNA

Volumn 6, Issue 11, 2000, Pages 1509-1515

  Mikheeva, Svetlana ^a   Murray, Heather L ^a   Zhou, Hui ^a   Turczyk, Brian M ^a   Jarrell, Kevin A ^a  

^a BOSTON UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Catalytic RNA; Group II intron; Ribozyme

Indexed keywords

ADENINE; DINUCLEOTIDE; GUANINE;

ARTICLE; DELETION MUTANT; EXON; GENE DELETION; GENE LOCUS; INTRON; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; RNA SPLICING; RNA STRUCTURE; STRUCTURE ANALYSIS;

ALTERNATIVE SPLICING; CLONING, MOLECULAR; CONSERVED SEQUENCE; DINUCLEOSIDE PHOSPHATES; EXONS; INTRONS; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; POINT MUTATION; RNA; RNA PRECURSORS; SEQUENCE DELETION; TRANSCRIPTION, GENETIC;

EID: 0033750527     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838200000972     Document Type: Article

References (17)

1. Studies of point mutants define three essential paired nucleotides in the domain 5 substructure of a group II intron
2. Interaction of intronic boundaries is required for the second splicing step efficiency of a group II intron
3. Catalytic site components common to both splicing steps of a group II intron
4. An RNA conformational change between the two chemical steps of group II self-splicing
5. Branch-point attack in group II introns is a highly reversible transesterification, providing a potential proofreading mechanism for 5'-splice site selection
6. Splice site selection and role of the lariat in a group II intron
7. Base-pairing interactions involving the 5' and 3'-terminal nucleotides of group II self-splicing introns
8. Group II intron domain 5 facilitates a trans-splicing reaction
9. Group II intron self-splicing. Alternative reaction conditions yield novel products
10. Group II introns deleted for multiple substructures retain self-splicing activity
11. Structure and activities of group II introns
12. Long-range intron-exon and intron-intron pairings involved in self-splicing of class II catalytic introns
13. Comparative and functional anatomy of group II catalytic introns - A review
14. Group II intron self-splicing: Development of alternative reaction conditions and identification of a predicted intermediate
15. Catalytically critical nucleotide in domain 5 of a group II intron
16. Stereochemical selectivity of group II intron splicing, reverse splicing, and hydrolysis reactions
17. Domain 5 binds near a highly conserved dinucleotide in the joiner linking domains 2 and 3 of a group II intron