Use of phosphosynapsin I-specific antibodies for image analysis of signal transduction in single nerve terminals

Journal of Cell Science

Volumn 113, Issue 20, 2000, Pages 3573-3582

  Menegon, A ^a   Dunlap, D D ^a   Castano, F ^a   Benfenati, F ^a   Czernik, A J ^a   Greengard, P ^a   Valtorta, F ^a  

^a SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

Ca2+; Cyclic AMP; Phosphorylation; Synapsin; Synaptic vesicle

Indexed keywords

CALCIUM ION; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; PROTEIN ANTIBODY; PROTEIN KINASE (CALCIUM,CALMODULIN); PROTEIN KINASE (CALCIUM,CALMODULIN) II; SYNAPSIN; SYNAPSIN I; SYNAPSIN II;

ANIMAL CELL; ANTIBODY LABELING; ARTICLE; CEREBELLUM; CONTROLLED STUDY; DEPHOSPHORYLATION; DEPOLARIZATION; ENZYME ACTIVATION; EXTRACELLULAR CALCIUM; GRANULAR CELL; IMAGE ANALYSIS; MOUSE; NERVE CELL; NERVE CELL CULTURE; NERVE ENDING; NERVE FIBER; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; QUANTITATIVE ASSAY; REGULATORY MECHANISM; SECOND MESSENGER; SIGNAL TRANSDUCTION; STOICHIOMETRY; SYNAPTOSOME;

ANIMALS; BUCLADESINE; CALCIUM; CELLS, CULTURED; CEREBELLUM; CYCLIC AMP; FLUORESCENT ANTIBODY TECHNIQUE; FORSKOLIN; IMMUNOBLOTTING; PHOSPHORYLATION; PRESYNAPTIC TERMINALS; RATS; RATS, SPRAGUE-DAWLEY; SECOND MESSENGER SYSTEMS; SIGNAL TRANSDUCTION; SYNAPSINS;

ANIMALIA;

EID: 0033747186     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Synapsin I bundles F-actin in a phosphorylation-dependent manner
2. Interaction of synapsin I with small synaptic vesicles: Distinct sites in synapsin I bind to vesicle phospholipids and vesicle proteins
3. Time-resolved fluorescence study of the neuron-specific phosphoprotein synapsin I. Evidence for phosphorylation-induced conformational changes
4. 2+/calmodulin-dependent protein kinase II is a binding protein for synapsin I
5. The end-plate potential in mammalian muscle
6. Focal adhesion kinase in rat central nervous system
7. 2+-dependent recycling of synaptic vesicles at the frog neuromuscular junction
8. Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I
9. Production of phosphorylation state-specific antibodies
10. The synapsins
11. A rapid Percoll gradient procedure for isolation of synaptosomes directly from an S1 fraction: Homogeneity and morphology
12. Glutamate receptor subtypes in cultured cerebellar neurons: Modulation of glutamate and gamma-aminobutyric acid release
13. Synaptic vesicle phosphoproteins and regulation of synaptic function
14. 2+ indicators with greatly improved fluorescence properties
15. Synapsins as regulators of neurotransmitter release
16. 2+
17. Multiple phosphorylation sites in protein I and their differential regulation by cyclic AMP and calcium
18. Differential phosphorylation of multiple sites in purified Protein I by cyclic AMP-dependent and calcium-dependent protein kinases
19. 2+/calmodulin-dependent cyclic nucleotide phosphodiesterase
20. Synaptic transmission: A bidirectional and self-modifiable form of cell-cell communication
21. Neurotrophins stimulate phosphorylation of synapsin I by MAP kinase and regulate synapsin I-actin interactions
22. A third member of the synapsin gene family
23. Release of acetylcholine from a nerve terminal by electric pulses of variable strength and duration
24. 2+/calmodulin-dependent, protein kinase II in hippocampal slices
25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
26. Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse
27. Site-specific phosphorylation of synapsin I by mitogen-activated protein kinase and Cdk5 and its effects on physiological functions
28. Tat protein from HIV-1 activates MAP kinase in granular neurons and glial cells from rat cerebellum
29. Visualization of the distribution of autophosphorylated calcium/calmodulin-dependent protein kinase II after tetanic stimulatio in the CA1 area of the hippocampus
30. Synapsin I: An actin-bundling protein under phosphorylation control
31. Immunochemical localization of calcium/calmodulin-dependent protein kinase I
32. Characterization of synapsin I binding to small synaptic vesicles
33. 2+/calmodulin kinase II inhibitor kN-62
34. Calcium ions, active zones and synaptic transmitter release
35. Synapsins: Mosaics of shared and individual domains in a family of synaptic vesicle phosphoproteins
36. Anti-synapsin monoclonal antibodies: Epitope mapping on mammalian synapsins using phage display libraries
37. Structure and function of the synapsins
38. Effects of the neuronal phosphoprotein synapsin I on actin polymerization
39. Membrane trafficking in nerve terminals
40. The synapsins and neurotransmission
41. Multiple factors regulating the release of norepinephrine consequent to nerve stimulation
42. Short-term synaptic plasticity