Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR

BioTechniques

Volumn 29, Issue 5, 2000, Pages 1012-1017

  Patrone, G ^a   Puppo, F ^a   Cusano, R ^a   Scaranari, M ^a   Ceccherini, I ^a   Puliti, A ^a   Ravazzolo, R ^a  

^a UNIVERSITY OF GENOA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN;

ARTICLE; CELL NUCLEUS; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; ISOTOPE LABELING; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA BINDING; RNA PURIFICATION; RNA SYNTHESIS; STATISTICAL ANALYSIS; TRANSCRIPTION REGULATION;

EID: 0033746028     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00295st02     Document Type: Article

References (13)

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5. Transcriptional regulation of hemoglobin switching in chicken embryos
6. Real time quantitative PCR
7. Nuclear runoff transcription analysis using chemiluminescent detection
8. Sequence and characterization of the RET proto-oncogene 5' flanking region: Analysis of retinoic acid responsiveness at the transcriptional level
9. Analysis of human interleukin-5 gene transcription by a novel nuclear ran-on method based on the polymerase chain reaction
10. Transcriptional repression by nucleosomes but not H1 in reconstituted preblastoderm Drosophila chromatin
11. A sensitive transcription assay based on simplified nuclear ran-on protocol
12. Scaffold/matrix-attached regions act upon transcription in a context-dependent manner
13. Expression of the RET proto-oncogene in human neuroblastoma cell lines and its increase during neuronal differentiation induced by retinoic acid