Visualization of nascent transcripts on Drosophila polytene chromosomes using BrUTP incorporation

BioTechniques

Volumn 29, Issue 5, 2000, Pages 934-936

  Chang, W Y ^a   Winegarden, N A ^a   Paraiso, J P ^a   Stevens, M L ^a   Westwood, J T ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; ECDYSONE; NUCLEOTIDE;

ARTICLE; CHROMOSOME ANALYSIS; DROSOPHILA MELANOGASTER; LARVA; POLYTENE CHROMOSOME; PROTEIN LOCALIZATION; SALIVARY GLAND; STAINING; TRANSCRIPTION REGULATION;

DROSOPHILA;

EID: 0033736999     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00295bm01     Document Type: Article

References (11)

1. Induction of Drosophila heat-shock puffs in isolated polytene nuclei
2. Polytene chromosome puffing and in situ hybridization measure different aspects of RNA metabolism
3. Labelling of eukaryotic transcripts with BrUTP using a non-liposomal transfection reagent
4. Labeling of RNA transcripts of eukaryotic cells in culture with BrUTP using a liposome transfection reagent (DOTAP)
5. Visualization of focal sites of transcription within human nuclei
6. Cytological localization of DNA complementary to ribisomal RNA in polytene chromosomes of diptera
7. Chromosomal synthesis of ribonucleic acid as shown by incorpration of uridine labeled with tritium
8. High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence
9. Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus
10. Stress-induced oligomerization and chromosomal relocalization of heat- shock factor