Diversity of phytoplasmas isolated from insects, determined by a DNA heteroduplex mobility assay and a length polymorphism of the 16S-23S rDNA spacer region analysis

Journal of Applied Microbiology

Volumn 89, Issue 5, 2000, Pages 744-750F

  Palmano, S ^a   Firrao, G ^a  

^a UNIVERSITY OF UDINE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

HETERODUPLEX; LENGTH POLYMORPHISM; PHYTOPLASMAS; PRESCREENING; REGION ANALYSIS; RELATIVE CONCENTRATION; RIBOSOMAL DNA; SPECIFIC PRIMERS;

POLYMERASE CHAIN REACTION;

NUCLEIC ACID; RIBOSOME DNA; SPACER DNA;

ARTICLE; BACTERIUM IDENTIFICATION; GENE AMPLIFICATION; GENETIC POLYMORPHISM; INSECT; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); HEXAPODA; INSECTA; MOLLICUTES; PHYTOPLASMA;

EID: 0033678773     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2000.01172.x     Document Type: Article

References (16)

1. Detection of plant pathogenic mycoplasma-like organisms' DNA by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene
2. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes
3. Isolation of plant DNA from fresh tissue
4. Molecular characterization of a phytoplasma causing phyllody in clover and other herbaceous hosts in Northern Italy
5. Production of oligonucleotide probes for mycoplasma-like organisms using the polymerase chain reaction
6. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients
7. Revised minimum standard for the description of new species of the class Mollicutes (division Tenericutes)
8. Detection of multiple phytoplasmas in perennial fruit trees with decline symptoms in Italy
9. Nonradioactive method to study genetic profiles of natural bacterial communities by PCR-Single-Strand-Conformation polymorphism
10. Detection of the Apple Proliferation and Pear Decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA
11. Plant diseases associated with mycoplasma-like organisms
12. Phylogenetic compositions of bacterioplankton from two California estuaries compared by denaturing gradient gel electrophoresis of 16S rDNA fragments
13. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA
14. PCR-induced (ligase-free) subcloning: A rapid reliable method to subclone polymerase chain reaction (PCR) products
15. Phytoplasma-specific PCR primers based on sequences of the 16/23S rRNA spacer region
16. Revised group classification of the genus Spiroplasma