Overexpression, purification, and in vitro refolding of the 11S globulin from amaranth seed in Escherichia coli

Journal of Agricultural and Food Chemistry

Volumn 48, Issue 11, 2000, Pages 5249-5255

  Osuna Castro, Juan A ^a   Rascón Cruz, Quintín ^a   Napier, Johnathan ^b   Fido, Roger J ^b   Shewry, Peter R ^b   Paredes López, Octavio ^a  

^a DEPARTAMENTO DE INGENIERÍA GENÉTICA   (Mexico)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Amarantin; Expression induction; Heterologous expression; Immunodetection; In vitro refolding; Recombinant amarantin; Storage protein

Indexed keywords

AMARANTH; AMINO ACID; AMMONIUM SULFATE; COMPLEMENTARY DNA; GLOBIN; GLOBULIN; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; IMMUNOCHEMISTRY; INTERMETHOD COMPARISON; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PHYTOCHEMISTRY; PLANT; PLANT SEED; PRECIPITATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; RESTRICTION MAPPING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GLOBULINS; PLANT PROTEINS; PLANTS, EDIBLE; PROTEIN FOLDING; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SEEDS;

AMARANTHUS CAUDATUS; ESCHERICHIA COLI; SPERMATOPHYTA;

EID: 0033672006     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf000795t     Document Type: Article

References (4)

1. Barba de la Rosa, A. P.; Gueguen, J,; Paredes-López, O.; Viroben, G. Fractionation procedures, electrophoretic characterization, and amino acid composition of amaranth seed proteins. J. Agric. Food Chem. 1992a, 40, 931-936.
2. Barba de la Rosa, A. P.; Paredes-López, O.; Gueguen, J. Characterization of amaranth globulins by ultraeentrifugation, Chromatographic techniques. J. Agric. Food Chem. 1992b, 40, 937-940.
3. Barba de la Rosa, A. P.; Herrera, A.; Utsumi, S.; Paredes-López, O. Molecular characterization, cloning and structural analysis of a cDNA encoding an amaranth globulin. J. Plant Physiol. 1996, 149, 527-532.
4. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.