Use of lactose to induce expression of soluble NifA protein domains of Herbaspirillum seropedicae in Escherichia coli

Canadian Journal of Microbiology

Volumn 46, Issue 11, 2000, Pages 1087-1090

  Monteiro, R A ^a   Souza, E M ^a   Yates, M G ^a   Pedrosa, F O ^a   Chubatsu, L S ^a  

^a FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

Author keywords

Herbaspirillum seropedicae; NifA protein; Nitrogen fixation; Protein expression; Transcriptional activator

Indexed keywords

BACTERIAL PROTEIN; DNA FRAGMENT; LACTOSE;

ARTICLE; CONTROLLED STUDY; DNA BINDING; ESCHERICHIA COLI; GENE OVEREXPRESSION; ISOTOPE LABELING; NITROGEN FIXATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SOLUBILITY;

ESCHERICHIA COLI; HERBASPIRILLUM SEROPEDICAE;

EID: 0033668824     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w00-094     Document Type: Article

References (15)

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3. 'Western Blotting': Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
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5. Genetic regulation of nitrogen fixation in rhizobia
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14. Increase of solubility of foreign proteins in Escherichia coli by co-production of the bacterial thioredoxin