The full-length, cytoplasmic C-terminus of the β2-adrenergic receptor expressed in E. coli acts as a substrate for phosphorylation by protein kinase A, insulin receptor tyrosine kinase, GRK2, but not protein kinase C and suppresses desensitization when expressed in vivo

Protein Expression and Purification

Volumn 20, Issue 3, 2000, Pages 451-461

  Doronin, Sergey ^a   Lin, Fubao ^a   Wang, Hsien Yu ^a   Malbon, Craig C ^a  

^a STONY BROOK UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

G proteinlinked receptor kinase; Insulin receptor tyrosine kinase; Protein kinase A; Protein kinase C; Protein phosphorylation; 2 Adrenergic receptor

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; CARCINOMA CELL; CELL LINE; CYCLIC AMP DEPENDENT PROTEIN KINASE; DISULFIDE BOND; ESCHERICHIA COLI; GENE MUTATION; GENETIC REGULATION; GUANINE NUCLEOTIDE BINDING PROTEIN; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN TYROSINE KINASE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 0033667975     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1322     Document Type: Article

References (38)

1. Isolation of phosphoproteins by immobilized metal (Fe3+) affinity chromatography
2. The beta-adrenergic receptor is a substrate for the insulin receptor tyrosine kinase
3. Functional desensitization of the isolated beta-adrenergic receptor by the beta-adrenergic receptor kinase: Potential role of an analog of the retinal protein arrestin (48-kDa protein)
4. Beta-adrenergic receptor kinase: Identification of a novel protein kinase that phosphorylates the agonist-occupied form of the receptor
5. Removal of phosphorylation sites from the beta 2-adrenergic receptor delays onset of agonist-promoted desensitization
6. G-protein-coupled receptors: Turn-ons and turn-offs
7. Agonist-promoted sequestration of the beta 2-adrenergic receptor requires regions involved in functional coupling with Gs
8. Identification of the G protein-coupled receptor kinase phosphorylation sites in the human beta2-adrenergic receptor
9. A dileucine motif in the C terminus of the beta2-adrenergic receptor is involved in receptor internalization
10. Role of clathrin-mediated endocytosis in agonist-induced down-regulation of the beta2-adrenergic receptor
11. Turning off the signal: Desensitization of beta-adrenergic receptor function
12. Two kinases mediate agonist-dependent phosphorylation and desensitization of the beta 2-adrenergic receptor
13. Crystal structure of the activated insulin receptor tyrosine kinase in complex with peptide substrate and ATP analog
14. Phosphorylation of tyrosyl residues 350/354 of the beta-adrenergic receptor is obligatory for counterregulatory effects of insulin
15. Insulin-like growth factor receptor-1 stimulates phosphorylation of the beta2-adrenergic receptor in vivo on sites distinct from those phosphorylated in response to insulin
16. G-protein-linked receptors as substrates for tyrosine kinases: Cross-talk in signaling
17. Insulin stimulates sequestration of beta-adrenergic receptors and enhanced association of beta-adrenergic receptors with Grb2 via tyrosine 350
18. The role of receptor kinases and arrestins in G protein-coupled receptor regulation
19. Truncation of the receptor carboxyl terminus impairs agonist-dependent phosphorylation and desensitization of the alpha 1B-adrenergic receptor
20. Altered patterns of agonist-stimulated cAMP accumulation in cells expressing mutant beta 2-adrenergic receptors lacking phosphorylation sites
21. Multiple pathways of rapid beta 2-adrenergic receptor desensitization. Delineation with specific inhibitors
22. G-protein-linked receptors as tyrosine kinase substrates: New paradigms in signal integration
23. Intramolecular disulfide bridges: Avenues to receptor activation
24. Physiological regulation of G protein-linked signaling
25. Mammalian beta 1- and beta 2-adrenergic receptors. Immunological and structural comparisons
26. Fat cell beta 1-adrenergic receptor: Structural evidence for existence of disulfide bridges essential for ligand binding
27. Beta-adrenergic receptors display intramolecular disulfide bridges in situ: Analysis by immunoblotting and functional reconstitution
28. Functional activation of beta-adrenergic receptors by thiols in the presence or absence of agonists
29. Mechanisms of acute desensitization of the beta2AR-adenylyl cyclase pathway in human airway smooth muscle
30. G protein-coupled receptor kinases
31. A key role for protein kinase A in homologous desensitization of the beta 2-adrenergic receptor pathway in S49 lymphoma cells
32. Beta 2-adrenergic receptor mutants reveal structural requirements for the desensitization observed with long-term epinephrine treatment
33. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
34. Dynamic complexes of beta2-adrenergic receptors with protein kinases and phosphatases and the role of gravin
35. Phosphorylation of the beta-adrenergic receptor in intact cells: Relationship to heterologous and homologous mechanisms of adenylate cyclase desensitization
36. Phosphorylation/dephosphorylation of the beta-adrenergic receptor regulates its functional coupling to adenylate cyclase and subcellular distribution
37. Homologous desensitization of adenylate cyclase is associated with phosphorylation of the beta-adrenergic receptor
38. cAMP-dependent protein kinase and protein kinase C consensus site mutations of the beta-adrenergic receptor. Effect on desensitization and stimulation of adenylylcyclase