Stability of fresh and preserved fetal and adult lymphocyte cell surface markers

Journal of Clinical Laboratory Analysis

Volumn 14, Issue 6, 2000, Pages 320-326

  Schumacher, Michael J ^a,b   Burkhead, Tamara ^a  

^a UNIVERSITY OF ARIZONA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF ARIZONA   (United States)

Author keywords

B lymphocytes; CD antigens; Flow cytometry; Lymphocytes; Methods; Preserving agents; Stability; T lymphocytes

Indexed keywords

CD19 ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD45 ANTIGEN; CD8 ANTIGEN; CELL SURFACE MARKER;

ANTIGEN EXPRESSION; ARTICLE; B LYMPHOCYTE; CRYOPRESERVATION; FLOW CYTOMETRY; GENETIC STABILITY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LYMPHOCYTE PRESERVATION; METHODOLOGY; T LYMPHOCYTE;

ADULT; ANTIGENS, CD; ANTIGENS, CD19; ANTIGENS, CD3; ANTIGENS, CD4; ANTIGENS, CD45; ANTIGENS, CD8; BIOLOGICAL MARKERS; BLOOD PRESERVATION; DRUG STABILITY; FEMALE; FETAL BLOOD; FETUS; FLOW CYTOMETRY; HUMANS; IMMUNOPHENOTYPING; LYMPHOCYTES; PREGNANCY; TIME FACTORS;

EID: 0033667566     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/1098-2825(20001212)14:6<320::AID-JCLA12>3.0.CO;2-W     Document Type: Article

References (10)

1. Quality assurance and immunophenotyping of lymphocytes, approved guidelines
2. The effects of anticoagulant and temperature on the measurements of helper and suppressor cells
3. Technical influences on immunophenotyping by flow cytometry. The effect of time and temperature of storage on the viability of lymphocyte subsets
4. Flow cytometry and cell sorting
5. Fixation and long term storage of human lymphocytes for surface marker analysis by flow cytometry
6. Holding medium for cell surface phenotypic analysis
7. Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells
8. Preservation of umbilical cord blood cells for immunophenotyping
9. Lymphocyte subset analysis by flow cytometry. Comparison of three different staining techniques and effects of blood storage