Double point mutant F34W/W140F of staphylococcal nuclease is in a molten globule state but highly competent to fold into a functional conformation

Journal of Biochemistry

Volumn 128, Issue 5, 2000, Pages 739-744

  Li, Y ^a   Jing, G ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

Author keywords

Folding; Ligands binding; Molten globule; Mutant; Staphylococcal nuclease

Indexed keywords

NUCLEASE;

ARTICLE; CIRCULAR DICHROISM; ENZYME SUBSTRATE COMPLEX; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; LIGAND BINDING; MICHAELIS MENTEN KINETICS; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE;

BACTERIA (MICROORGANISMS);

EID: 0033667255     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022810     Document Type: Article

References (37)

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12. The crystal structure of staphylococcal nuclease refined at 1.7 Å resolution
13. 2+, and the inhibitor pdTp, refined at 1.65 Å
14. Folding of staphylococcal nuclease A studied by equilibrium and kinetic circular dichroism spectra
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17. Cold denaturation of staphylococcal nuclease
18. Calculation of protein extinction coefficients from amino acid sequence data
19. Conformational features of a truncated staphylococcal nuclease R (SNR135) and their implications for catalysis
20. Use of fast protein size-exclusion liquid chromatography to study the unfolding of proteins which denature through the molten globule
21. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus
22. 2+-liganding amino acid in staphylococcal nuclease
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24. Structural characterization of the molten globule of α-lactalbumin by solution X-ray scattering
25. Study of the 'molten globule' intermediate state in protein folding by a hydrophobic fluorescent probe
26. Truncated staphylococcal nuclease is compact but disordered
27. Mapping the structure of a non-native state of staphylococcal nuclease
28. An in vitro peptide folding model suggests the presence of the molten globule state during nascent peptide folding
29. Residual structure in a staphylococcal nuclease fragment. Is it a molten globule and is its unfolding a first-order phase transition?
30. NMR analysis of the residual structure in the denatured state of an unusual mutant of staphylococcal nuclease
31. The phase transition between a compact denatured state and a random coil state in staphylococcal nuclease is first-order
32. Three-state thermodynamic analysis of the denaturation of staphylococcal nuclease mutants
33. Compact state of a protein molecule with pronounced small-scale mobility: Bovine alpha-lactalbumin
34. α-Lactalbumin: Compact state with fluctuating tertiary structure?
35. Forcing thermodynamically unfolded proteins to fold
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37. Folding funnels, binding funnels, and protein function