Fusion expression of Escherichia coli PrlC gene and preparation of PrlC proteinase affinity column

Biotechnology Letters

Volumn 22, Issue 22, 2000, Pages 1741-1745

  Jiang, Xueyuan ^a   Jin, Zhicheng ^a   Cheng, Ni ^a   Zhu, Dexu ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Affinity column; E. coli; Fusion expression; PrlC proteinase

Indexed keywords

HYBRID PROTEIN; NICKEL; RESIN; TRYPTASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL CYCLE; GENE EXPRESSION; HYDROLYSIS; MOLECULAR CLONING; PROKARYOSIS;

EID: 0033663551     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005607000906     Document Type: Article

References (12)

1. Cloning and nucleotide sequence of opdA, the gene encoding oligopepitdase A in Salmonella typhbnurium
2. Escherichia coli prlC encodes an endopeptidase and is homologous to Salmonella typhimurium opdA gene
3. Oligopepitdase A is required for normal phage P22 development
4. Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations
5. Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle
6. A trypsin inhibitor trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester suppresses the onset of DNA synthesis in Escherichia coli cells synchronized by phosphate starvation
7. Purification and characterization of proteinase In, a trypsin-like proteinase, in Escherichia coli
8. Rapid isolation of high-molecular-weight plant DNA
9. Localization and purification of two enzymes from Escherichia coli capable of hydrolyzing a signal peptide
10. Genetic analysis of protein export in Escherichia coli
11. Characterization and in vivo cloning of prlC, a suppressor of signal sequence mutations in Escherichia coli K12
12. PrlC, a suppressor of signal sequence mutations in Escherichia coli, can direct the insertion of the signal sequence into the membrane