Comparison of different methods for the detection of anti-Ro/SSA antibodies in connective tissue diseases

Clinical and Experimental Rheumatology

Volumn 18, Issue 6, 2000, Pages 729-731

  Morozzi, G ^a   Bellisai, F ^a   Simpatico, A ^a   Pucci, G ^a   Bacarelli, M R ^a   Campanella, V ^a   Marcolongo, R ^a   Galeazzi, M ^a  

^a UNIVERSITY OF SIENA   (Italy)

Author keywords

Anti Ro antibodies; Methods; Sensitivity; Specificity

Indexed keywords

AUTOANTIBODY;

ANTIBODY DETECTION; ARTICLE; COMPARATIVE STUDY; CONNECTIVE TISSUE DISEASE; CONTROLLED STUDY; DEVICE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNODIFFUSION; IMMUNOFLUORESCENCE; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; SJOEGREN SYNDROME; SYSTEMIC LUPUS ERYTHEMATOSUS;

ANTIBODIES, ANTINUCLEAR; BLOTTING, WESTERN; CONNECTIVE TISSUE DISEASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; IMMUNODIFFUSION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY;

EID: 0033656605     PISSN: 0392856X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (15)

1. Biochemical and immunological heterogeneity of the Ro ribonucleoprotein particles. Analysis with sera specific for the Ro hY5 particle
2. Human Ro ribonucleoprorein particles: Characterization of native structare and stable association with the La polypeptide
3. Purification of antigenically intact Ro ribonucleoproteins; biochemical and immunological evidence that the 52-kd-protein is not a Ro protein
4. Molecular composition of Ro small ribonucleoprotein complexes in human cells: Intracellular localization of the 60- and 52-kD proteins
5. Protein heterogeneity in the human Ro/SSA ribonucleoproteins: The 52- and 60-kd Ro/SSA autoantigens are encoded by separate genes
6. Autoantibodies to the Ro/ SSA antigen are conformation dependent; autoantibodies to the denatured form of 52 kDa Ro/SSA are crossreacting subset of antibodies to the native 60kDaRo/SSA molecule
7. Ro ribonucleoprotein assembly in vitro: Identification of RNA-protein and protein-protein interactions
8. Epitope specificity determines the ability of anti-Ro 52 autoantibodies to precipitate Ro ribonucleoprotein particles
9. Calreticulin binds hY-RNA and the 52-kDa polypeptide component of the Ro SS-A ribonucleoprotein autoantigen
10. Fine specificity of autoimmune response to Ro/SSA and La/SSB ribonucleoproteins
11. RO (SS-A) and anti-RO (SS-A): An update
12. A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities. I. Precision, sensitivity, and specificity
13. Autoantibody testing for connective tissue diseases. Comparison of immunodiffusion, immunoblot, and enzyme immunoassay
14. Counterimmuno-electrophoresis, ELISA and immunoblotting detection of anti-Ro/SSA antibodies in subacute cutaneous lupus erythematosus. A comparative study
15. Detecting anti-SSA and anti-SSB antibodies in routine analysis: A comparison between double immunodiffusion and immanoblotting